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Here we show that pH is the most important parameter determining the effect of ageing on zinc partitioning in soils, with the effect of ageing becoming more important with increasing pH. Furthermore, zinc bioavailability, expressed as the internal zinc concentrations in red clover (<i>Trifolium pratense<\/i>) is closely related to pore water zinc concentration. In addition, there is a clear dose-response relationship between the survival of the earthworm <i>Eisenia fetida<\/i> and the calcium chloride-extracted zinc fraction. These results indicate that zinc partitioning can be used to predict zinc bioavailability to terrestrial organisms. 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