{"refrec":{"BRefID":12910,"RR":"<b>Osborne, W.K.; Verhaaf, E.</b> (1979). Toxische en sublethale effekten van koper en zink bij <i>Arenicola marina</i> en de invloed van koper en zink op de zuurstofaffiniteit van het hemoglobine van <i>Arenicola marina</i>. <i>Interne verslagen Nederlands Instituut voor Onderzoek der Zee</i>, 1979(16). NIOZ: Texel. 32 pp.","BEntID":12910,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":". <i>Interne verslagen Nederlands Instituut voor Onderzoek der Zee</i>, 1979(16). NIOZ: Texel.  32 pp.","DocTypID":13,"DocType":"Reports","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Osborne, W.K.; Verhaaf, E.","OrigTitleTranslFlag":1,"Authorstringtrunc":"Osborne, W.K.; Verhaaf, E.","Englishabstract":"The toxicity of Cu<sup>2+</sup> and Zn<sup>2+</sup> in concentrations of respectively 2-40 ppm and 10-200 ppm for the lugworm <i>Arenicola marina</i> L. was determined under static conditions (pH 7.70; 15°C). The values of Lt<sub>50</sub> for the different concentrations of Cu<sup>2+</sup> and Zn<sup>2+</sup> vary between 47 and 129 hours, the values of Lc<sub>50</sub> (96 hr) are 1.6 ppm for Cu<sup>2+</sup> and 136 ppm for Zn<sup>2+</sup>. Different kinds of visible sublethal effects are observed. There was no mortality during the whole experiment (max. 186 hr) for specimen in blanco medium (pH 7.70) and for animals in medium with different activity of H<sub>3</sub>O<sup>+</sup> (pH 6.35; 6.70; 7.10). The quantity of toxicant in the medium, measured at the end of the experiment by anodic stripping voltametry, was less than the quantity that was added to the medium at the start of the experiment. The oxygen affinity of a solution of <i>Arenicola</i> haemoglobin in which the concentration of Cu<sup>2+</sup> or Zn<sup>2+</sup> was 5.10<sup>-7</sup> -5.10<sup>-3</sup>M was determined using an oxygen diffusion chamber technique. The O<sub>2</sub>-partial pressures at halfsaturation (p<sub>50</sub>) are 6.5 mm Hg to 0.22 mm Hg (pH 6.6; 7.0; 7.5; 8.1; 15°C) The Bohr-factor varied from -0.59 for pure <i>Arenicola</i> haemoglobin to -0.38 for haemoglobin with Cu<sup>2+</sup> in a concentration of 5.10<sup>-4</sup> M and to -0,42 for the same concentration of Zn<sup>2+</sup>. The results are discussed in relation to the measured environmental parameters.","AbstractOtherLang":"De toxiciteit van Cu<sup>2+</sup> en Zn<sup>2+</sup> in concentraties van respectievelijk 2-40 ppm en 10-200 ppm is onder statische omstandigheden onderzocht voor <i>Arenicola marina</i> L. (pH 7,70; 15°C). De Lt<sub>50</sub>-waarden voor verschillende concentraties Cu<sup>2+</sup> en Zn<sup>2+</sup> liggen tussen 47 en 129 uur en Lc<sub>50</sub> (96 hr)-waarden zijn 1,6 ppm voor Cu<sup>2+</sup> en 136 ppm voor Zn<sup>2+</sup>. Verschillende zichtbare sublethale effecten zijn waargenomen. Bij proefdieren in blanco medium (pH 7,70) en in media met verschillende H<sub>3</sub>O<sup>+</sup>-aktiviteit (pH 6,35; 6,70; 7,10) trad geen mortaliteit op gedurende het gehele experiment (maximaal 186 uur). De achteraf, door middel van anodische heroplossingsvoltametrie bepaalde concentraties Cu<sup>2+</sup> en Zn<sup>2+</sup> in het medium, zijn lager dan die welke bij aanvang van het experiment aan het medium werden toegevoegd. De zuurstofaffiniteit van een oplossing van <i>Arenicola marina</i> waarin Cu<sup>2+</sup> en Zn<sup>2+</sup> in concentraties van 5.10<sup>-7</sup> tot en met 5.10<sup>-3</sup> molair aanwezig waren, werd gemeten met behulp van de zuurstofdiffusiekamertechniek. De halfverzadigingsspanningen (p<sub>50</sub>-waarden) liggen tussen 6,5 mm Hg en 0,22 mm Hg bij pH 6,6; 7,0; 7,5 en 8,1 (15°C). De Bohr-factor verandert van -0,59 voor zuiver <i>Arenicola marina</i> hemoglobine tot -0,38 voor hemoglobine met een concentratie van 5.10<sup>-5</sup> molair Cu<sup>2+</sup>, en tot -0,42 voor eenzelfde concentratie Zn<sup>2+</sup>. De resultaten worden besproken in relatie tot de gemeten milieuparameters.","BibLvlCode":"MS","StandardTitle":"Toxische en sublethale effekten van koper en zink bij <i>Arenicola marina</i> en de invloed van koper en zink op de zuurstofaffiniteit van het hemoglobine van <i>Arenicola marina</i>","OrigTitleLangCode":"nl","OrigTitleLangCodeExtended":"dut","OrigTitleLangID":41,"DateLastModified":{"date":"2025-07-22 01:33:06.175990","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":null,"MonPub":1979,"DateUpdate":"2025-07-15","DateCreate":"2001-07-05","SecASFANote":null,"ConfID":null,"PeerRev":0,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1},"refs":null,"anarec":null,"monrec":{"MonID":12910,"ISBN":null,"PubliDate":1979,"IssueDate":null,"Volume":"1979","Issue":"16","Pagination":"32","Place":"Texel","Edition":null,"BRefXtra":null,"BRefXtraRR":null,"SerID":5036,"SerRR":"Interne verslagen Nederlands Instituut voor Onderzoek der Zee. 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