{"refrec":{"BRefID":13069,"RR":"<b>Budding, M.C.</b> (1974). De scheiding van slib en plankton. <i>NIOZ-rapport</i>, 1974(4). Nederlands Instituut voor Onderzoek der Zee: Texel. 38 pp.","BEntID":13069,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":". <i>NIOZ-rapport</i>, 1974(4). Nederlands Instituut voor Onderzoek der Zee: Texel.  38 pp.","DocTypID":13,"DocType":"Reports","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Budding, M.C.","OrigTitleTranslFlag":1,"Authorstringtrunc":"Budding, M.C.","Englishabstract":"It is possible to separate non-living suspended matter and living plankton with the help of a common laboratory centrifuge and a commercial silica-gel called LUDOX. With this method it becomes possible to determine particle size of suspended matter and plankton separately with e.g. a Coulter Counter. The following method has been used. First the particle size distribution of the original (mixed) sample is determined with the Coulter Counter. Then an aliquot is centrifuged in order to concentrate the particles in a smaller volume. Then the concentrated sample is brought into a centrifuge phial that contains a 22% sucrose-solution on top of a layer of LUDOX. The sample is centrifuged during ten minutes with a velocity of 3500 rpm. After this, the non-living particles are concentrated on the bottom of the phial whereas the plankton is concentrated on top of the LUDOX. The plankton is carefully collected and resuspended in (filtered) seawater. Counting this plankton in the Coulter Counter gives the size distribution as it was in the original sample. The size distribution of non-living particles concentrated on the bottom of the phial will in most cases not give the original size distribution: complex processes of flocculation and aggregation of clay particles and organic matter probably will take place during concentration and centrifuging, making it necessary to use an indirect method.","AbstractOtherLang":"Met behulp van een gewone laboratorium-centrifuge en het in de handel verkrijgbare silica-gel LUDOX is het mogelijk in zeewater gesuspendeerd slib en plankton van elkaar te scheiden. Daardoor wordt het mogelijk de grootteverdeling van de slib-en planktondeeltjes afzonderlijk te bepalen met behulp van automatische (i.c. Coulter Counter-)tellingen. Allereerst wordt daartoe het gesuspendeerde materiaal, nog in het oorspronkelijke monster verenigd, geteld. Vervolgens wordt het de eerste keer gecentrifugeerd in het \"eigen\" zeewater, om het te concentreren. Daarna wordt het geconcentreerde materiaal in een centrifugebuis gebracht, op een laagje 22% sucrose-oplossing, dat zich op zijn beurt op een laag LUDOX bevindt. De buis wordt gedurende 10 minuten met een snelheid van 3500 toeren per minuut gecentrifugeerd, waarna het slib zich op de bodem van de buis bevindt, en het plankton op het oppervlak van de LUDOX rust. Dit plankton wordt zeer voorzichtig teruggewonnen, en opnieuw in (gefiltreerd) zeewater gesuspendeerd. Telling van deze suspensie geeft de grootteverdeling van het plankton, zoals het in het oorspronkelijke monster aanwezig was. De grootteverdeling van het slib wordt gevonden door de waarden die de laatste telling heeft opgeleverd af te trekken van de waarden uit de eerste telling. Directe telling van de slibdeeltjes onderuit de centrifugebuis levert zeer waarschijnlijk niet de grootteverdeling van het slib zoals het in het oorspronkelijke monster voorkwam op. Het complexe flocculatie-gedrag van de kleimineralen, waaruit het slib grotendeels bestaat, maakt indirecte telling noodzakelijk.","BibLvlCode":"MS","StandardTitle":"De scheiding van slib en plankton","OrigTitleLangCode":"nl","OrigTitleLangCodeExtended":"dut","OrigTitleLangID":41,"DateLastModified":{"date":"2025-07-22 01:33:06.175990","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":null,"MonPub":1974,"DateUpdate":"2025-07-15","DateCreate":"2001-07-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1},"refs":null,"anarec":null,"monrec":{"MonID":13069,"ISBN":null,"PubliDate":1974,"IssueDate":null,"Volume":"1974","Issue":"4","Pagination":"38","Place":"Texel","Edition":null,"BRefXtra":null,"BRefXtraRR":null,"SerID":5697,"SerRR":"NIOZ-rapport. Netherlands Institute for Sea Research (NIOZ): Den Burg.  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