{"refrec":{"BRefID":13081,"RR":"<b>de Wilde, H.</b> (1974). De O<sub>2</sub> bindende eigenschappen van het haemoglobine en aspecten van Hb-heterogeniteit van <i>Pleuronectus platessa</i> L. en <i>Pleuronectus flesus</i> L. <i>Interne verslagen Nederlands Instituut voor Onderzoek der Zee</i>, 1974(5). Nederlands Instituut voor Onderzoek der Zee: Texel. 36 pp.","BEntID":13081,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":". <i>Interne verslagen Nederlands Instituut voor Onderzoek der Zee</i>, 1974(5). Nederlands Instituut voor Onderzoek der Zee: Texel.  36 pp.","DocTypID":13,"DocType":"Reports","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"de Wilde, H.","OrigTitleTranslFlag":1,"Authorstringtrunc":"de Wilde, H.","Englishabstract":"1. A comparative study was made of the oxygenation properties of haemoglobine (Hb) from plaice <i>Pleuronectus platessa</i> L. and of flounder (<i>Pleuronectus flesus</i> L.)2. The multiple haemoglobins in each species were separated by means of iso-electric focusing and oxygen binding properties of the main component haemoglobins were determined. 3. The oxygen affinity of whole blood, measured directly after collection is higher in plaice than in flounder, under the same conditions of pH and temperature. Removal of the blood plasma lowers the oxygen affinity of the haemoglobin in the erythrocytes. This shift is less pronounced in the flounder than in plaice and corresponds with dilution effects of the Hb and intracellular factors such as the organic phosphates 4. The isolated Hb components of plaice showed small mutual differences in oxygen affinity at the same pH's and temperatures. These differences were not found to be significant.5. The Bohr effect for the washed red cells and for Hb in solution of plaice and flounder, and for the six main components of plaice. The Bohr effect, expressed as Delta log P<sub>50</sub>/Delta pH, of plaice erythrocytes and of Hb in solution were -0,64 and -0,78 respectively; for flounder the corresponding values were -0,53 and -0,45. The Bohr factor for the six components of plaice Hb varied from -1,26 to -1,43.6. The overall temperature influence, expressed in terms of the apparent heat of oxygenation, delta H, amounted to -1,14 and -16,6 kcal/mole respectively for erythrocytes and Hb solution of plaice. For flounder the corresponding values found were -15,1 and -19,1 kcal/mole. For five of the main Hb components of plaice the delta H values varied from -14,5 to -17,0 kcal/mole.","AbstractOtherLang":"1. Een vergelijkende studie werd gemaakt van de zuurstofbindende eigenschappen van haemoglobine van schol<i>Pleuronectes platessa</i> L. en bot (<i>Pleuronectus flesus</i> L.)2. Met behulp van een preparatieve electrofocusing techniek zijn de Hb-componenten van bot en schol gescheiden voor het bepalen van de zuurstofbindende eigenschappen van de voornaamste componenten.3. De zuurstofaffiniteit van onbehandeld bloed, direct na bemonstering gemeten, is voor schol hoger dan voor bot, onder gelijke omstandigheden van temperatuur en pH. Het verwijderen van het bloedplasma heeft bij schol en bij bot in mindere mate, verlaging van de zuurstofaffiniteit van Hb in de erythrocyten tot gevolg. Haemolyse van de erythrocyten veroorzaakt een verhoging van de zuurstofaffiniteit van Hb in de erythrocyten tot gevolg. Haemolyse van de erythrocyten veroorzaakt een verhoging van de zuurstofaffiniteit van het Hb. Dit hangt samen met een verdunningseffect van Hb en ook van intracellulaire factoren zoals organische fosfaten.4. De gescheiden Hb componenten van schol vertonen onderling kleine verschillen in zuurstofaffiniteit onder gelijke omstandigheden van temperatuur en pH. Deze verschillen zijn echter niet significant.5. Het Bohr effect is bepaald van gewassen erythrocyten en Hb in oplossing van schol en bot en van de zes voornaamste Hb-componenten van schol.De Bohr-effect index delta log P<sub>50</sub>/Delta pH, voor schol bedraagt voor de erythrocyten en Hb in oplossing respectievelijk -0,64 en -0,78; voor bot respectievelijk -0,53 en -0,45. De Bohr-effect index voor zes verschillende Hb-componenten van schol variëert van -1,26 tot -1,43.6. Het temperatuur-effect is bepaald van gewassen erythrocyten en Hb in oplossing van schol en bot en van vijf Hb componenten van schol. Het totale temperatuur effect, uitgedrukt als de ogenschijnlijke oxygenatiewarmte, delta H, bedraagt respectievelijk -1,14 and -16,6 kcal/mole voor schol-erythrocyten en Hb in oplossing. Voor bot zijn overeenkomende waarden gevonden van-15,1 and -19,1 kcal/mole. Voor vijf van de voornaamste Hb componenten van schol varieert de delta H waarde van -14,5 to -17,0 kcal/mole.","BibLvlCode":"MS","StandardTitle":"De O<sub>2</sub> bindende eigenschappen van het haemoglobine en aspecten van Hb-heterogeniteit van <i>Pleuronectus platessa</i> L. en <i>Pleuronectus flesus</i> L","OrigTitleLangCode":"nl","OrigTitleLangCodeExtended":"dut","OrigTitleLangID":41,"DateLastModified":{"date":"2025-07-22 01:33:06.175990","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":null,"MonPub":1974,"DateUpdate":"2025-07-15","DateCreate":"2001-07-10","SecASFANote":null,"ConfID":null,"PeerRev":0,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1},"refs":null,"anarec":null,"monrec":{"MonID":13081,"ISBN":null,"PubliDate":1974,"IssueDate":null,"Volume":"1974","Issue":"5","Pagination":"36","Place":"Texel","Edition":null,"BRefXtra":null,"BRefXtraRR":null,"SerID":5036,"SerRR":"Interne verslagen Nederlands Instituut voor Onderzoek der Zee. 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