{"refrec":{"BRefID":131582,"RR":"<b>Kime, D.E.; Lee, E.Y.; Kim, H.S.</b> (2009). Cloning and characterization of alginate lyase from a marine bacterium <i>Streptomyces</i> sp. ALG-5. <i>Mar. Biotechnol. 11(1)</i>: 10-16. <a href=\"https://dx.doi.org/10.1007/s10126-008-9114-9\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-008-9114-9</a>","BEntID":125632,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Biotechnol. 11(1)</i>: 10-16. <a href=\"https://dx.doi.org/10.1007/s10126-008-9114-9\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-008-9114-9</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Kime, D.E.; Lee, E.Y.; Kim, H.S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Kime, D.E.; Lee, E.Y.; Kim, H.S.","Englishabstract":"A marine bacterium was isolated from seaweeds for its ability to degrade alginate. Analysis of 16S ribosomal DNA sequence and chemotaxonomic characterizations revealed that the strain belongs to <i>Streptomyces</i>. The alginate lyase gene of <i>Streptomyces</i> sp. ALG-5 was cloned by using PCR with the specific primer designed from homologous nucleotide sequences. The consensus sequences of N-terminal YXRSELREM and C-terminal YFKAGXYXQ were conserved in the ALG-5 alginate lyase gene. The recombinant alginate lyase was purified by using Ni-Sepharose affinity chromatography. The alginate lyase appears to be poly-guluronate lyase degrading poly-G block preferentially than poly-M block. The degraded products were determined to be di-, tri-, tetra- and pentasaccharides by using BioGel P-2 gel filtration chromatography and ionization mass spectroscopy method. ","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Cloning and characterization of alginate lyase from a marine bacterium <i>Streptomyces</i> sp. 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