{"refrec":{"BRefID":133774,"RR":"<b>Truby, E.W.</b> (1997). Preparation of single-celled marine dinoflagellates for electron microscopy. <i>Microsc. Res. Tech. 36(4)</i>: 337-340","BEntID":127730,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Microsc. Res. Tech. 36(4)</i>: 337-340","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Truby, E.W.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Truby, E.W.","Englishabstract":"Electron microscopy has been used successfully to study and identify single-celled marine dinoflagellates including parasitic ones and others, such as those that cause red tide. Delicate cells can be preserved for scanning electron microscopy with a combined glutaraldehydeosmium tetroxide mixture that is adjusted for the osmolality of the medium. The protocol allows resolution of fine morphological features. Preservation for transmission electron microscopy can be accomplished with a standard glutaraldehyde fixation and osmium-tetroxide post-fixation in a suitable buffer, but again, the osmolality of the mixture must be adjusted. The protocol allows ultrastructural resolution of vesiculated cells and has been modified for small sample sizes","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Preparation of single-celled marine dinoflagellates for electron microscopy","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1997,"MonPub":null,"DateUpdate":"2009-04-15","DateCreate":"2009-04-15","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1997WV93300011","VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":133774,"PubliDate":1997,"Pagination":"337-340","XtraPublOfAnaID":null,"ISBN":null,"Volume":"36","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":133772,"SerRR":"Microscopy Research and Technique. Wiley-Liss: New York, NY.  ISSN 1059-910X; e-ISSN 1097-0029","StandardTitleSer":"Microscopy Research and Technique","ISSN":"1059-910X","AbbrevSer":"Microsc. Res. Tech.","StandardTitleMon":null,"StartPage":337,"Pages":4,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Truby","Firstname":"Earnest","Initials":"E.W.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":127730,"AutID":53141,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":null,"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Wiley-Liss","City":"New York, NY"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Morphology","ThestID":101436,"Acronym":"TRT","ThesTermPath":"Morphology"}],"thestermsASFA":[{"ThesaurusTerm":"Morphology"}],"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":127730,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":127730,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":null,"thesterms":[{"ThesaurusTerm":"Morphology","ThestID":101436,"Acronym":"TRT","ThesTypID":3,"ThesType":"Transportation Research Thesaurus"}],"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Morphology","DutchTerm":"Morfologie","ThestID":101436,"Acronym":"TRT","ThesTypID":3,"ThesType":"Transportation Research Thesaurus"}],"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Dewitte, Elien, E.","newSesDate":{"date":"2009-04-15 11:15:04.120000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Dewitte, Elien, E.","updSesDate":{"date":"2009-04-15 11:15:04.120000","timezone_type":3,"timezone":"Europe/Brussels"}}}