{"refrec":{"BRefID":133884,"RR":"<b>Brown Jr., A.</b> (1971). Experimental techniques for preserving diatoms used as food for larval <i>Penaeus aztecus</i>, <b><i>in</i></b>: <i>Proceedings of the National Shellfisheries Association 62. Proceedings of the National Shellfisheries Association,</i> 62: pp. 21-25","BEntID":127834,"PublicFlag":1,"CheckedFlag":0,"wosflag":null,"vabbflag":null,"RefStringPartII":", <b><i>in</i></b>: <i>Proceedings of the National Shellfisheries Association 62. Proceedings of the National Shellfisheries Association,</i> 62: pp. 21-25","DocTypID":17,"DocType":"Book chapters","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Brown Jr., A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Brown Jr., A.","Englishabstract":"The diatoms <i>Skeletonema costatum</i> and <i>Thalassiosira</i> sp were freeze-dried and fed to larval brown shrimp, <i>P. aztecus</i>, being reared to 1st stage mysis from 1st stage protozoa. Feeding levels ranged from 100,000-1,000,000 cells/ml. Both diatoms were also frozen, either in the presence or absence of the protectants glycerol and dimethyl sulfoxide. Frozen <i>Skeletonema</i> was suitable when frozen in the presence of protectants; <i>Thalassiosira</i> supported good growth of larval shrimp when frozen alone or with protectants. 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