{"refrec":{"BRefID":134064,"RR":"<b>Wang, B.; Li, F.; Luan, W.; Xie, Y.; Zhang, C.; Luo, Z.; Gui, L.; Yan, H.; Xiang, J.</b> (2008). Comparison of gene expression profiles of <i>Fenneropenaeus chinensis</i> challenged with WSSV and <i>Vibrio</i>. <i>Mar. Biotechnol. 10(6)</i>: 664-675. <a href=\"https://dx.doi.org/10.1007/s10126-008-9105-x\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-008-9105-x</a>","BEntID":127994,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Biotechnol. 10(6)</i>: 664-675. <a href=\"https://dx.doi.org/10.1007/s10126-008-9105-x\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-008-9105-x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Wang, B.; Li, F.; Luan, W.; Xie, Y.; Zhang, C.; Luo, Z.; Gui, L.; Yan, H.; Xiang, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Wang, B. <i>et al.</i>","Englishabstract":"Microarray technique was used to analyze the gene expression profiles of shrimp when they were challenged by WSSV and heat-inactivated <i>Vibrio anguillarum</i>, respectively. At 6 h post challenge (HPC), a total of 806 clones showed differential expression profile in WSSV-challenged samples, but not in <i>Vibrio</i>-challenged samples. The genes coding energy metabolism enzyme and structure protein were the most downregulated elements in 6 h post WSSV-challenged (HPC-WSSV) tissues. However, a total of 155 clones showed differential expression in the <i>Vibrio</i>-challenged samples, but not in WSSV-challenged samples. Serine-type endopeptidase and lysosome-related genes were the most upregulated elements in tissues 6 h post <i>Vibrio</i> challenge (HPC-Vibrio). Totally, 188 clones showed differential expression in both 6 and 12 HPC-WSSV and HPC-<i>Vibrio</i> samples. Most of the differentially expressed genes (185/188) were downregulated in the samples of 12 HPC-WSSV, whereas upregulated in the samples at 6 and 12 HPC-<i>Vibrio</i> and 6 HPC-WSSV. The expression profiles of three differentially expressed genes identified in microarray hybridization were analyzed in hemocytes, lymphoid organ, and hepatopancreas of shrimp challenged by WSSV or <i>Vibrio</i> through real-time PCR. The results further confirmed the microarray hybridization results. 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