{"refrec":{"BRefID":134073,"RR":"<b>Shah, D.H.; Cain, K.D.; Wiens, G.D.; Call, D.R.</b> (2008). Challenges associated with heterologous expression of <i>Flavobacterium psychrophilum</i> proteins in <i>Escherichia coli</i>. <i>Mar. Biotechnol. 10(6)</i>: 719-730. <a href=\"https://dx.doi.org/10.1007/s10126-008-9111-z\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-008-9111-z</a>","BEntID":128002,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Biotechnol. 10(6)</i>: 719-730. <a href=\"https://dx.doi.org/10.1007/s10126-008-9111-z\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-008-9111-z</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Shah, D.H.; Cain, K.D.; Wiens, G.D.; Call, D.R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Shah, D.H. <i>et al.</i>","Englishabstract":"A two-parameter statistical model was used to predict the solubility of 96 putative virulence-associated proteins of <i>Flavobacterium psychrophilum</i> (CSF259-93) upon over expression in <i>Escherichia coli</i>. This analysis indicated that 88.5% of the <i>F. psychrophilum</i> proteins would be expressed as insoluble aggregates (inclusion bodies). These solubility predictions were verified experimentally by colony filtration blot for six different <i>F. psychrophilum</i> proteins. A comprehensive analysis of codon usage identified over a dozen codons that are used frequently in <i>F. psychrophilum</i>, but that are rarely used in <i>E. coli</i>. Expression of <i>F. psychrophilum</i> proteins in <i>E. coli</i> was often associated with production of minor molecular weight products, presumably because of the codon usage bias between these two organisms. Expression of recombinant protein in the presence of rare tRNA genes resulted in marginal improvements in the expressed products. Consequently, <i>Vibrio parahaemolyticus</i> was developed as an alternative expression host because its codon usage is similar to <i>F. psychrophilum</i>. A full-length recombinant <i>F. psychrophilum</i> hemolysin was successfully expressed and purified from <i>V. parahaemolyticus</i> in soluble form, whereas this protein was insoluble upon expression in <i>E. coli</i>. We show that <i>V. parahaemolyticus</i> can be used as an alternate heterologous expression system that can remedy challenges associated with expression and production of <i>F. psychrophilum</i> recombinant proteins.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Challenges associated with heterologous expression of <i>Flavobacterium psychrophilum</i> proteins in <i>Escherichia coli</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:31:36.864333","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2008,"MonPub":null,"DateUpdate":"2020-10-30","DateCreate":"2009-04-21","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000260838600010","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10126-008-9111-z"},"refs":null,"anarec":{"AnaID":134073,"PubliDate":2008,"Pagination":"719-730","XtraPublOfAnaID":null,"ISBN":null,"Volume":"10","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45426,"SerRR":"Marine Biotechnology. 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