{"refrec":{"BRefID":134847,"RR":"<b>Vandenbrouck, T.; Soetaert, A.; Van der Ven, K.; Blust, R.; De Coen, W.</b> (2009). Nickel and binary metal mixture responses in <i>Daphnia magna</i>: molecular fingerprints and (sub)organismal effects. <i>Aquat. Toxicol. 92(1)</i>: 18-29. <a href=\"http://dx.doi.org/10.1016/j.aquatox.2008.12.012\" target=\"_blank\">dx.doi.org/10.1016/j.aquatox.2008.12.012</a>","BEntID":128779,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Toxicol. 92(1)</i>: 18-29. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2008.12.012\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2008.12.012</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Vandenbrouck, T.; Soetaert, A.; Van der Ven, K.; Blust, R.; De Coen, W.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Vandenbrouck, T. <i>et al.</i>","Englishabstract":"The recent development of a custom cDNA microarray platform for one of thé standard organisms in aquatic toxicology, <i>Daphnia magna</i>, opened up new ways to mechanistic insights of toxicological responses. In this study, the mRNA expression of several genes and (sub)organismal responses (Cellular Energy Allocation, growth) were assayed after short-term waterborne metal exposure. Microarray analysis of Ni-exposed daphnids revealed several affected functional gene classes, of which the largest ones were involved in different metabolic processes (mainly protein and chitin related processes), cuticula turnover, transport and signal transduction. Furthermore, transcription of genes involved in oxygen transport and heme metabolism (haemoglobin, d-aminolevilunate synthase) was down-regulated. Applying a Partial Least Squares regression on nickel fingerprints and biochemical (sub)organismal parameters revealed a set of co-varying genes (haemoglobin, RNA terminal phosphate cyclase, a ribosomal protein and an “unknown” gene fragment). An inverse relationship was seen between the mRNA expression levels of different cuticula proteins and available energy reserves. In addition to the nickel exposure, daphnids were exposed to binary mixtures of nickel and cadmium or nickel and lead. Using multivariate analysis techniques, the mixture mRNA expression fingerprints (Ni<sup>2+</sup> + Cd<sup>2+</sup>, Ni<sup>2+</sup> + Pb<sup>2+</sup>) were compared to those of the single metal treatments (Ni<sup>2+</sup>, Cd<sup>2+</sup>, Pb<sup>2+</sup>). It was hypothesized that the molecular fingerprints of the mixtures would be additive combinations of the gene transcription profiles of the individual compounds present in the mixture. However, our results clearly showed additionally affected pathways after mixture treatment (e.g. additional affected genes involved in carbohydrate catabolic processes and proteolysis), indicating interactive molecular responses which are not merely the additive sum of the individual metals. These findings, although indicative of the complex nature of mixture toxicity evaluation, underline the potential of a toxicogenomics approach in gaining more mechanistic information on the effects of single compounds and mixtures.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Nickel and binary metal mixture responses in <i>Daphnia magna</i>: molecular fingerprints and (sub)organismal effects","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Daphnia magna; Gene transcription; mRNA expression; Cellular Energy Allocation; Nickel; Cadmium; Lead; Binary metal mixtures","OtherDescriptors":null,"Notes":null,"AnaPub":2009,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2009-05-05","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000265154900003","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2008.12.012"},"refs":null,"anarec":{"AnaID":134847,"PubliDate":2009,"Pagination":"18-29","XtraPublOfAnaID":null,"ISBN":null,"Volume":"92","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. 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