{"refrec":{"BRefID":136995,"RR":"<b>Keenan, K.; Erlich, E.; Donnelly, K.H.; Basel, M.B.; Hutner, S.H.; Kassoff, R.; Crawford, S.A.</b> (1978). Particle-based axenic media for tetrahymenids. <i>J. Protoz. 25(3)</i>: 385-387. <a href=\"http://dx.doi.org/10.1111/j.1550-7408.1978.tb03911.x\" target=\"_blank\">http://dx.doi.org/10.1111/j.1550-7408.1978.tb03911.x</a>","BEntID":130816,"PublicFlag":1,"CheckedFlag":0,"wosflag":null,"vabbflag":1,"RefStringPartII":". <i>J. Protoz. 25(3)</i>: 385-387. <a href=\"http://dx.doi.org/10.1111/j.1550-7408.1978.tb03911.x\" target=\"_blank\">http://dx.doi.org/10.1111/j.1550-7408.1978.tb03911.x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Keenan, K.; Erlich, E.; Donnelly, K.H.; Basel, M.B.; Hutner, S.H.; Kassoff, R.; Crawford, S.A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Keenan, K. <i>et al.</i>","Englishabstract":"Autoclavable, natural particulate media simplify axenic cultivation of tetrahymenid ciliates and presumably favor selection for phagotrophy. Viability is at least 2 months at room temperature (24-26 C) for the lipid-sensitive tetrahymenids <i>Tetrahymena setosa, T. corlissi, T. paravorax, T. limacis, and T. patula</i>, also for <i>T. rostrata</i> and (at 12 C), for strains of the <i>T. pyriformis</i> complex and <i>Glaucoma chattoni</i>. A typical medium consists of crude soy \"lecithin\" + skim milk powder + <i>Saccharomyces cerevisiae</i> cells. Other useful particules readily available commercially are: whole liver powder, cells of <i>Micrococcus lysodeikticus</i> and <i>Escherichia coli</i>, and powdered residue of liver which had been extracted with 70% ethanol (\"liver No. 2). Preliminary experiments indicate that some of these media are suitable for the maintenance of <i>Paramecium octaurelia</i> stock 299S and <i>Colpidium campylum</i>. Such mixtures may serve as points of departure for devising media for more fastidious phagotrophs.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Particle-based axenic media for tetrahymenids","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-14 01:31:18.610243","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Tetrahymena spp.;phagotrophy;particulate maintenance media;Paramecium octaurelia;Glaucoma chattoni;Colpidium campylum.","OtherDescriptors":null,"Notes":null,"AnaPub":1978,"MonPub":null,"DateUpdate":"2017-01-31","DateCreate":"2009-06-05","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1978FU02700021","VABBcode":null,"OpenAcc":0,"DOI":"10.1111/j.1550-7408.1978.tb03911.x"},"refs":null,"anarec":{"AnaID":136995,"PubliDate":1978,"Pagination":"385-387","XtraPublOfAnaID":null,"ISBN":null,"Volume":"25","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":119190,"SerRR":"The Journal of Protozoology. 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