{"refrec":{"BRefID":137561,"RR":"<b>D'Agostino, A.S.; Provasoli, L.</b> (1970). Dixenic culture of <i>Daphnia magna</i> Straus. <i>Biol. Bull. 139</i>: 485-494. <a href=\"https://dx.doi.org/10.2307/1540367\" target=\"_blank\">https://dx.doi.org/10.2307/1540367</a>","BEntID":131329,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Biol. Bull. 139</i>: 485-494. <a href=\"https://dx.doi.org/10.2307/1540367\" target=\"_blank\">https://dx.doi.org/10.2307/1540367</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":1,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"D'Agostino, A.S.; Provasoli, L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"D'Agostino, A.S.; Provasoli, L.","Englishabstract":"1. <i>Daphnia magna</i> can be grown for at least 200 generations, axenically or in crude cultures, in a defined mineral medium, enriched with vitamin B12 and thiamine and 1 ml/100 of a dilute organic medium, when fed with <i>Chlamydomonas reinhardi</i> and <i>Scenedesmus obliquus</i>.2. The organic enrichment is essential for maintaining continued fertility of <i>D. magna</i>.3. The organic enrichment can be replaced by the addition of pantothenic acid to vitamin B12 and thiamine without lowering the fertility of <i>D. magna</i>.4. The organic enrichment (or the vitamin mixture) does not act directly on <i>D. magna</i> but via the algae by changing their nutritional value for <i>Daphina</i>.5. 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