{"refrec":{"BRefID":138025,"RR":"<b>Ewing, R.D.; Peterson, G.L.; Conte, F.P.</b> (1974). Larval salt gland of<i>Artemia salina</i> nauplii. Localization and characterization of the sodium+potassium-activated Adenosine Triphosphatase. <i>J. Comp. Physiol. A Sens. Neural Behav. Physiol. 88(3)</i>: 217-234","BEntID":131756,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>J. Comp. Physiol. A Sens. Neural Behav. Physiol. 88(3)</i>: 217-234","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ewing, R.D.; Peterson, G.L.; Conte, F.P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ewing, R.D.; Peterson, G.L.; Conte, F.P.","Englishabstract":"The naupliar stage of <i>Artemia salina</i> has been found to contain large amounts of an ATPase which requires the presence of Mg<sup>++</sup>, is stimulated by Na<sup>+</sup> and K<sup>+</sup>, and is sensitive to ouabain. Upon cell fractionation, sixty percent of the Na+K-activated ATPase activity was present in a heavy membrane fraction which accompanied mitochondria, and twenty percent of the activity was found in the microsomal fraction. Separation of the heavy membranes containing the Na+K-activated ATPase from mitochondria was accomplished by sucrose density gradient centrifugation.Kinetic characterization of the Na+K-activated ATPase was examined with both mitochondrial and microsomal preparations. No significant differences were observed between the two preparations. Maximal enzyme activity occurred at pH 7.2 and a temperature of 45 ° C. Half maximal inhibition of enzymatic activity occurred with ouabain concentration of 8 × 10<sup>-6</sup> M. Half maximal activation of the enzyme by ATP and MgCl<sub>2</sub> occurred at 8 × 10<sup>-4</sup> and 1 × 10<sup>-3</sup> M, respectively, with an optimal Mg<sup>++</sup>/ATP ratio of 2.0. NaCl and KCl (or NH<sub>4</sub>Cl) were required for activity, with half maximal activation at 1 × 10<sup>-2</sup> and 9 × 10<sup>-3</sup> M (or 2.1 × 10<sup>-2</sup> M), respectively. The optimal Na<sup>+</sup>/K<sup>+</sup> ratio was 4.0, although enzymatic activity occurred through a wide range of ratios. In contrast, survival of nauplii was reduced in media in which the Na<sup>+</sup>/K<sup>+</sup> ratio was less than 10.Anatomical localization of the enzyme in nauplii indicated that the abdominal region contained 44% of the total Na+K-activated ATPase activity, while the cephalothoracic region contained 54% of the total activity. Isolated larval salt glands contained 10% or less of the total activity. In contrast, the adult leg segments containing the salt-transporting organs showed 54% of the total Na+K-activated ATPase activity.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Larval salt gland of<i>Artemia salina</i> nauplii. Localization and characterization of the sodium+potassium-activated Adenosine Triphosphatase","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1974,"MonPub":null,"DateUpdate":"2009-06-19","DateCreate":"2009-06-19","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":138025,"PubliDate":1974,"Pagination":"217-234","XtraPublOfAnaID":null,"ISBN":null,"Volume":"88","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43221,"SerRR":"Journal of Comparative Physiology A. Sensory, Neural, and Behavioral Physiology. Springer: Heidelberg; Berlin.  ISSN 0340-7594; e-ISSN 1432-1351","StandardTitleSer":"Journal of Comparative Physiology A. Sensory, Neural, and Behavioral Physiology","ISSN":"0340-7594","AbbrevSer":"J. Comp. Physiol. A Sens. Neural Behav. Physiol.","StandardTitleMon":null,"StartPage":217,"Pages":18,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Ewing","Firstname":null,"Initials":"R.D.","Affiliation":null,"Discriminator":null,"CorporateFlag":1,"BEntID":131756,"AutID":88049,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Peterson","Firstname":null,"Initials":"G.L.","Affiliation":null,"Discriminator":null,"CorporateFlag":1,"BEntID":131756,"AutID":88050,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Conte","Firstname":null,"Initials":"F.P.","Affiliation":null,"Discriminator":null,"CorporateFlag":1,"BEntID":131756,"AutID":88051,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":null,"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Springer","City":"Heidelberg; Berlin"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":131756,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":131756,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":null,"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Dewitte, Elien, E.","newSesDate":{"date":"2009-06-19 09:27:08.127000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Dewitte, Elien, E.","updSesDate":{"date":"2009-06-19 09:27:08.127000","timezone_type":3,"timezone":"Europe/Brussels"}}}