{"refrec":{"BRefID":140460,"RR":"<b>Marty, G.D.; Bower, S.M.; Clarke, K.R.; Meyer, G.; Lowe, G.; Osborn, A.L.; Chow, H.; Byrne, S.; Sojonky, K.; Robinson, J.H.</b> (2006). Histopathology and a real-time PCR assay for detection of <i>Bonamia ostreae</i> in <i>Ostrea edulis</i> cultured in western Canada. <i>Aquaculture 261(1)</i>: 33-42. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2006.07.024\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2006.07.024</a>","BEntID":133942,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquaculture 261(1)</i>: 33-42. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2006.07.024\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2006.07.024</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Marty, G.D.; Bower, S.M.; Clarke, K.R.; Meyer, G.; Lowe, G.; Osborn, A.L.; Chow, H.; Byrne, S.; Sojonky, K.; Robinson, J.H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Marty, G.D. <i>et al.</i>","Englishabstract":"<i>Bonamia ostreae</i> is an intracellular haplosporidian parasite in European flat oysters <i>Ostrea edulis</i> that occurs on both coasts of the United States and causes significant mortality in Europe. Canada was considered free of <i>B. ostreae</i> until 2004, when it was first detected in <i>O. edulis</i> obtained for laboratory study from a western Canadian oyster farm. <i>Bonamia ostreae</i> was confirmed in <i>O. edulis</i> at the index farm in November 2004 using histopathology, conventional polymerase chain reaction (PCR) assays, restriction fragment length polymorphism (RFLP) analysis, and sequencing of the PCR product. Archived samples of European flat oysters obtained from the index farm between 1999 and 2004 (n=343) were re-examined and all samples collected before 2003 (n=306) were confirmed negative for <i>B. ostreae</i> by histopathology (n=306) and PCR (n=62). In archived samples from 2003, <i>B. ostreae</i> was detected in 3 of 37 <i>O. edulis</i> by histopathology (n=2) and/or PCR (n=2). Also, records indicate that <i>B. ostreae</i> was not detected in <i>O. edulis</i> (n=348) from five other locations in western Canada between 1986 and 2000. To better understand the distribution and prevalence of <i>B. ostreae</i> in western Canada, 607 oysters from the index farm and 2 additional farms were sampled in the summer of 2005. All 3 farms had been stocked with <i>O. edulis</i> spat from the State of Washington, USA, where <i>B. ostreae</i> is endemic. Samples were analyzed by histopathology and a new real-time PCR that amplifies a 68-bp target DNA fragment. <i>B. ostreae</i> was detected in all three locations, with prevalence ranging from 0.5 to 11.1%. Diagnostic sensitivity of the real-time PCR method was consistently greater than histopathology. Also, preliminary evidence supports the conclusion that real-time PCR on paraffin sections is more sensitive than histopathology;<i> B. ostreae</i> DNA was confirmed in 4 oysters by real-time PCR on paraffin-embedded tissues (and independently confirmed on unfixed tissues) that was not detected by histopathology. As a result of these findings, <i>O. edulis</i> spat are no longer allowed to be imported from endemic areas into Canada.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Histopathology and a real-time PCR assay for detection of <i>Bonamia ostreae</i> in <i>Ostrea edulis</i> cultured in western Canada","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2006,"MonPub":null,"DateUpdate":"2014-07-03","DateCreate":"2009-08-13","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000242738100005","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquaculture.2006.07.024"},"refs":null,"anarec":{"AnaID":140460,"PubliDate":2006,"Pagination":"33-42","XtraPublOfAnaID":null,"ISBN":null,"Volume":"261","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42190,"SerRR":"Aquaculture. Elsevier: Amsterdam; London; New York; Oxford; Tokyo.  ISSN 0044-8486; e-ISSN 1873-5622","StandardTitleSer":"Aquaculture","ISSN":"0044-8486","AbbrevSer":"Aquaculture","StandardTitleMon":null,"StartPage":33,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Marty","Firstname":"Gary","Initials":"G.D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":133942,"AutID":24721,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bower","Firstname":"Susan","Initials":"S.M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":133942,"AutID":13222,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Clarke","Firstname":"K. Robert","Initials":"K.R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":133942,"AutID":79968,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Meyer","Firstname":null,"Initials":"G.","Affiliation":null,"Discriminator":null,"CorporateFlag":1,"BEntID":133942,"AutID":89148,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Lowe","Firstname":null,"Initials":"G.","Affiliation":null,"Discriminator":null,"CorporateFlag":1,"BEntID":133942,"AutID":89149,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Osborn","Firstname":null,"Initials":"A.L.","Affiliation":null,"Discriminator":null,"CorporateFlag":1,"BEntID":133942,"AutID":89150,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Chow","Firstname":"Hannah","Initials":"H.","Affiliation":null,"Discriminator":null,"CorporateFlag":1,"BEntID":133942,"AutID":89151,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Byrne","Firstname":null,"Initials":"S.","Affiliation":null,"Discriminator":null,"CorporateFlag":1,"BEntID":133942,"AutID":89152,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Sojonky","Firstname":null,"Initials":"K.","Affiliation":null,"Discriminator":null,"CorporateFlag":1,"BEntID":133942,"AutID":89153,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Robinson","Firstname":null,"Initials":"J.H.","Affiliation":null,"Discriminator":null,"CorporateFlag":1,"BEntID":133942,"AutID":89154,"OrderNr":10,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":258799,"LibID":36,"BRefID":140460,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"258799","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"Amsterdam; London; New York; Oxford; Tokyo"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTermPath":"Acids > Organic compounds > Organic acids > Nucleic acids > DNA"},{"ThesaurusTerm":"Marine aquaculture","ThestID":4986,"Acronym":"ASFA","ThesTermPath":"Aquaculture > Marine aquaculture"},{"ThesaurusTerm":"Archives","ThestID":9635,"Acronym":"ASFA","ThesTermPath":"Archives"},{"ThesaurusTerm":"Oyster culture","ThestID":5943,"Acronym":"ASFA","ThesTermPath":"Cultures > Shellfish culture > Mollusc culture > Oyster culture"},{"ThesaurusTerm":"Spat","ThestID":7851,"Acronym":"ASFA","ThesTermPath":"Developmental stages > Larvae > Invertebrate larvae > Molluscan larvae > Spat"},{"ThesaurusTerm":"Farms","ThestID":79078,"Acronym":"CSA","ThesTermPath":"Farms"},{"ThesaurusTerm":"Nucleotide sequence","ThestID":9567,"Acronym":"ASFA","ThesTermPath":"Nucleotide sequence"},{"ThesaurusTerm":"Paraffin","ThestID":100037,"Acronym":"TRT","ThesTermPath":"Paraffin"},{"ThesaurusTerm":"Parasites","ThestID":6001,"Acronym":"ASFA","ThesTermPath":"Parasites"},{"ThesaurusTerm":"Histopathology","ThestID":4014,"Acronym":"ASFA","ThesTermPath":"Pathology > Histopathology"},{"ThesaurusTerm":"Polymerase chain reaction","ThestID":9583,"Acronym":"ASFA","ThesTermPath":"Polymerase chain reaction"},{"ThesaurusTerm":"Mortality","ThestID":5352,"Acronym":"ASFA","ThesTermPath":"Population functions > Mortality"},{"ThesaurusTerm":"Endemic species","ThestID":2764,"Acronym":"ASFA","ThesTermPath":"Taxa > Species > Endemic species"},{"ThesaurusTerm":"Coasts","ThestID":1658,"Acronym":"ASFA","ThesTermPath":"Topographic features > Landforms > Coasts"}],"thestermsASFA":[{"ThesaurusTerm":"Archives"},{"ThesaurusTerm":"Coasts"},{"ThesaurusTerm":"DNA"},{"ThesaurusTerm":"Endemic species"},{"ThesaurusTerm":"Farms"},{"ThesaurusTerm":"Histopathology"},{"ThesaurusTerm":"Marine aquaculture"},{"ThesaurusTerm":"Mortality"},{"ThesaurusTerm":"Nucleotide sequence"},{"ThesaurusTerm":"Oyster culture"},{"ThesaurusTerm":"Paraffin"},{"ThesaurusTerm":"Parasites"},{"ThesaurusTerm":"Polymerase chain reaction"},{"ThesaurusTerm":"Spat"}],"taxtermsASFA":[{"TaxTerm":"Bonamia ostreae"},{"TaxTerm":"Ostrea edulis Linnaeus, 1758"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":133942,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":133942,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.aquaculture.2006.07.024","externalID":"10.1016/j.aquaculture.2006.07.024","URLTypeCode":"DOI","URLID":110303,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Archives","ThestID":9635,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Coasts","ThestID":1658,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Endemic species","ThestID":2764,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Farms","ThestID":79078,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Histopathology","ThestID":4014,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Marine aquaculture","ThestID":4986,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Mortality","ThestID":5352,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Nucleotide sequence","ThestID":9567,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Oyster culture","ThestID":5943,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Paraffin","ThestID":100037,"Acronym":"TRT","ThesTypID":3,"ThesType":"Transportation Research Thesaurus"},{"ThesaurusTerm":"Parasites","ThestID":6001,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Polymerase chain reaction","ThestID":9583,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Spat","ThestID":7851,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":[{"TaxTerm":"Bonamia ostreae","AphiaID":246871,"TaxtID":36904},{"TaxTerm":"Ostrea edulis Linnaeus, 1758","AphiaID":140658,"TaxtID":17006}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Archives","DutchTerm":"Archieven","ThestID":9635,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Coasts","DutchTerm":"Kusten","ThestID":1658,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"DNA","DutchTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Endemic species","DutchTerm":null,"ThestID":2764,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Farms","DutchTerm":null,"ThestID":79078,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Histopathology","DutchTerm":null,"ThestID":4014,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Marine aquaculture","DutchTerm":null,"ThestID":4986,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Mortality","DutchTerm":"Sterfte","ThestID":5352,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Nucleotide sequence","DutchTerm":null,"ThestID":9567,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Oyster culture","DutchTerm":null,"ThestID":5943,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Paraffin","DutchTerm":null,"ThestID":100037,"Acronym":"TRT","ThesTypID":3,"ThesType":"Transportation Research Thesaurus"},{"ThesaurusTerm":"Parasites","DutchTerm":"Parasieten","ThestID":6001,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Polymerase chain reaction","DutchTerm":"Polymerase kettingreactie","ThestID":9583,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Spat","DutchTerm":null,"ThestID":7851,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":[{"TaxTerm":"Bonamia ostreae","DutchTerm":"oesterparasiet","AphiaID":246871,"TaxtID":36904},{"TaxTerm":"Ostrea edulis Linnaeus, 1758","DutchTerm":"Eetbare oester","AphiaID":140658,"TaxtID":17006}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Dewitte, Elien, E.","newSesDate":{"date":"2009-08-13 08:58:43.580000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2014-07-03 08:35:34.640000","timezone_type":3,"timezone":"Europe/Brussels"}}}