{"refrec":{"BRefID":142268,"RR":"<b>Fitzpatrick, M.S.; Gale, W.L.; Schreck, C.B.</b> (1994). Binding characteristics of an androgen receptor in the ovaries of coho salmon, <i>Oncorhynchus kisutch</i>. <i>Gen. Comp. Endocrinol. 95</i>: 399-408. <a href=\"https://dx.doi.org/10.1006/gcen.1994.1139\" target=\"_blank\">https://dx.doi.org/10.1006/gcen.1994.1139</a>","BEntID":135488,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Gen. Comp. Endocrinol. 95</i>: 399-408. <a href=\"https://dx.doi.org/10.1006/gcen.1994.1139\" target=\"_blank\">https://dx.doi.org/10.1006/gcen.1994.1139</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Fitzpatrick, M.S.; Gale, W.L.; Schreck, C.B.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Fitzpatrick, M.S.; Gale, W.L.; Schreck, C.B.","Englishabstract":"Androgen receptors were identified in the cytosol from ovaries of juvenile coho salmon, Oncorhynchus kisutch. The binding for the synthetic androgen mibolerone was specific and saturable (K<sub>d</sub> = 0.32 plus or minus 0.02 nM; B<sub>max</sub> = 15.31 plus or minus 4.31 fmol/mg protein). Bound [<sup>3</sup>H]mibolerone was much higher in ovarian cytosol than in cytosolic extracts from heart, liver, and muscle. [ <sup>3</sup>H]mibolerone specific binding was 50% lower in the plasma than in the ovarian cytosolic extracts. [ <sup>3</sup>H]mibolerone binding was displaced most effectively by those 17 alpha -methylated synthetic androgens (mibolerone, methyltestosterone, methylandrostanolone) that can induce functional masculinization in fish. The naturally occurring androgens 11-ketotestosterone and 5 alpha -dihydrotestosterone both displaced [<sup>3</sup>H]mibolerone binding, but they were 10- to 100-fold less effective than the 17 alpha -methylated androgens. Testosterone, 11 beta -hydroxyandrostenedione, estradiol, progesterone, and 17 alpha ,20 beta -dihydroxyprogesterone were not potent competitors. [ <sup>3</sup>H]mibolerone specific binding was reduced after preincubation with trypsin. About 25% of the binding in the cytosolic extract had DNA binding affinity under experimental conditions. The characteristics of this androgen binding site are consistent with a model of receptor-mediated steroid-induced sex inversion.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Binding characteristics of an androgen receptor in the ovaries of coho salmon, <i>Oncorhynchus kisutch</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-04 01:31:02.586589","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1994,"MonPub":null,"DateUpdate":"2009-11-24","DateCreate":"2009-11-24","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1994PH31800011","VABBcode":null,"OpenAcc":0,"DOI":"10.1006/gcen.1994.1139"},"refs":null,"anarec":{"AnaID":142268,"PubliDate":1994,"Pagination":"399-408","XtraPublOfAnaID":null,"ISBN":null,"Volume":"95","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42729,"SerRR":"General and Comparative Endocrinology. 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