{"refrec":{"BRefID":143236,"RR":"<b>Vandegehuchte, M.B.; Kyndt, T.; Vanholme, B.; Haegeman, A.; Gheysen, G.; Janssen, C.R.</b> (2009). Occurrence of DNA methylation in <i>Daphnia magna</i> and influence of multigeneration Cd exposure. <i>Environ. Int. 35(4)</i>: 700-706. <a href=\"https://dx.doi.org/10.1016/j.envint.2009.01.002\" target=\"_blank\">https://dx.doi.org/10.1016/j.envint.2009.01.002</a>","BEntID":136422,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Environ. Int. 35(4)</i>: 700-706. <a href=\"https://dx.doi.org/10.1016/j.envint.2009.01.002\" target=\"_blank\">https://dx.doi.org/10.1016/j.envint.2009.01.002</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Vandegehuchte, M.B.; Kyndt, T.; Vanholme, B.; Haegeman, A.; Gheysen, G.; Janssen, C.R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Vandegehuchte, M.B. <i>et al.</i>","Englishabstract":"Most of the research on the epigenetic phenomenon of DNA methylation has been performed with vertebrates and plants. Knowledge on DNA methylation in <i>Daphnia magna</i>, a key test organism in aquatic toxicology, is completely lacking. Through epigenetic inheritance, effects of transient chemical exposure could be transferred to non-exposed generations, which could have a major impact on ecological risk assessment procedures. In this study, we determined if CpG methylation occurs in <i>D. magna</i> and if this can be influenced by exposure to toxic substances. Homologs of human DNA methyltransferases DNMT1, DNMT2 and DNMT3A were found in the partially available <i>D. magna</i> genome. Using an optimized “Amplification of Intermethylated Sites (AIMS)” technique, two methylated fragments were discovered in <i>D. magna</i> DNA. No homology was found for these sequences. The methylation and the <i>D. magna</i> origin of the fragments were confirmed with Southern analysis. This optimized AIMS technique was then applied to DNA of <i>D. magna</i> which were exposed to 180 µg/L Cd for two generations. Exposure resulted in a significant decrease in reproduction. The same methylated fragments with the same band intensity were observed in DNA of both non-exposed and exposed daphnids. As such, it could not be demonstrated that Cd exposure altered DNA methylation. However, the presence of DNA methylation in <i>D. magna</i> shows that potentially epigenetic effects may occur in this species.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Occurrence of DNA methylation in <i>Daphnia magna</i> and influence of multigeneration Cd exposure","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Epigenetics; DNA methylation; Invertebrate; Ecotoxicology; Daphniamagna; CpG methylation","OtherDescriptors":null,"Notes":null,"AnaPub":2009,"MonPub":null,"DateUpdate":"2019-06-27","DateCreate":"2010-01-20","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000265520300004","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.envint.2009.01.002"},"refs":null,"anarec":{"AnaID":143236,"PubliDate":2009,"Pagination":"700-706","XtraPublOfAnaID":null,"ISBN":null,"Volume":"35","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42659,"SerRR":"Environment International. 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