{"refrec":{"BRefID":143239,"RR":"Vandegehuchte, M.B.; Lemière, F.; Janssen, C.R. (2009). Quantitative DNA-methylation in Daphnia magna and effects of multigeneration Zn exposure. Comp. Biochem. Physiol. C-Toxicol. Pharmacol. 150(3): 343-348. https://dx.doi.org/10.1016/j.cbpc.2009.05.014","BEntID":136425,"PublicFlag":1,"CheckedFlag":1,"wosflag":0,"vabbflag":null,"RefStringPartII":". Comp. Biochem. Physiol. C-Toxicol. Pharmacol. 150(3): 343-348. https://dx.doi.org/10.1016/j.cbpc.2009.05.014","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Vandegehuchte, M.B.; Lemière, F.; Janssen, C.R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Vandegehuchte, M.B. et al.","Englishabstract":"Little research on the epigenetic phenomenon of DNA methylation has been performed with invertebrates. However, a few studies have shown that effects of transient chemical exposure can be transferred to non-exposed generations through epigenetic inheritance. If this is a wide-spread phenomenon it may have major consequences for current ecological risk assessments. The presence of DNA methylation in Daphnia magna has only very recently been demonstrated, although not quantified. In the present study, the overall content of 5-methyl-2′-deoxycytidine (5mdC) in the DNA of D. magna was determined. Additionally, the effect of transgenerational Zn exposure on the 5mdC content was assessed. An optimized LC-MS-MS method proved to be very suitable for measuring overall levels of 5mdC in relatively small samples of D. magna DNA. The average [5mdC]/[dG] ratio ranged from 0.13% to 0.81%. A slight but significant decrease in DNA methylation was found in the offspring (F1) of Zn exposed daphnids in the parental generation. However, this effect did not propagate into the next generation (F2). The presence of DNA methylation in D. magna and the finding that different exposure histories can entail different levels of methylation, open the way for further investigations on the implications of epigenetics in aquatic toxicology.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Quantitative DNA-methylation in Daphnia magna and effects of multigeneration Zn exposure","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-18 01:31:29.485735","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Epigenetics; Daphnia magna; Cytosine methylation; Ecotoxicology","OtherDescriptors":null,"Notes":null,"AnaPub":2009,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2010-01-20","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000269231400003","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.cbpc.2009.05.014"},"refs":null,"anarec":{"AnaID":143239,"PubliDate":2009,"Pagination":"343-348","XtraPublOfAnaID":null,"ISBN":null,"Volume":"150","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":128736,"SerRR":"Comparative Biochemistry and Physiology. C. Comparative Pharmacology and Toxicology. Pergamon: Oxford; New York. ISSN 0742-8413; e-ISSN 1878-1942","StandardTitleSer":"Comparative Biochemistry and Physiology. C. Comparative Pharmacology and Toxicology","ISSN":"0742-8413","AbbrevSer":"Comp. Biochem. Physiol. C-Toxicol. Pharmacol.","StandardTitleMon":null,"StartPage":343,"Pages":6,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Vandegehuchte","Firstname":"Michiel","Initials":"M.B.","Affiliation":"Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":136425,"AutID":259284,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"GhEnToxLab","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Milieutoxicologie","ORCID":"0000-0002-3915-4416","PersID":10749,"InsID":6705},{"AutName":"Lemière","Firstname":"Filip","Initials":"F.","Affiliation":"Nucleoside Research and Mass Spectrometry Unit, Centre for Proteome Analysis and Mass Spectrometry (CeProMa), Department of Chemistry, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":136425,"AutID":316712,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Janssen","Firstname":"Colin","Initials":"C.R.","Affiliation":"Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":136425,"AutID":144240,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"GhEnToxLab","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Milieutoxicologie","ORCID":"0000-0002-7781-6679","PersID":50,"InsID":6705}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":311466,"LibID":36,"BRefID":143239,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"311466","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"
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