{"refrec":{"BRefID":143657,"RR":"<b>Wang, P.; Wang, G.; Teng, Y.; Li, X.; Ji, J.; Xu, X.; Li, Y.</b> (2010). Effects of cefotaxime and kanamycin on thallus proliferation and differentiation in <i>Porphyra yezoensis</i> and their inhibition on <i>Agrobacterium tumefaciens</i>. <i>Mar. Biol. Res. 6(1)</i>: 100-105. <a href=\"https://dx.doi.org/10.1080/17451000902933017\" target=\"_blank\">https://dx.doi.org/10.1080/17451000902933017</a>","BEntID":136828,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Biol. Res. 6(1)</i>: 100-105. <a href=\"https://dx.doi.org/10.1080/17451000902933017\" target=\"_blank\">https://dx.doi.org/10.1080/17451000902933017</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Wang, P.; Wang, G.; Teng, Y.; Li, X.; Ji, J.; Xu, X.; Li, Y.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Wang, P. <i>et al.</i>","Englishabstract":"The effects of two antibiotics such as cefotaxime and kanamycin on growth and differentiation of <i>Porphyra yezoensis</i> thalli and their inhibition on <i>Agrobacterium</i> <i>tumefaciens</i> were evaluated in order to develop an efficient technique for <i>Agrobacterium</i>-mediated genetic transformation. Cefotaxime at more than 500 mg/l and 300 mg/l resulted in suppression of <i>P</i>. <i>yezoensis</i> thallus growth and the formation of conchocelis, respectively, but kanamycin had no affects on <i>P</i>. <i>yezoensis</i> thallus growth at concentrations of 100-600 mg/l. The inhibition of antibiotics on <i>P</i>. <i>yezoensis</i> thallus differentiation was observed at cefotaxime concentration of more than 300 mg/l and kanamycin concentration of more than 600 mg/l, respectively, and no conchocelis were formed at these concentrations. Both of antibiotics possessed strong inhibition on <i>A</i>. <i>tumefaciens</i> growth. Cefotaxime at 200 mg/l significantly inhibited <i>A</i>. <i>tumefaciens</i> strains LBA4404 and AGL growth. Almost complete kanamycin suppressions on <i>A</i>. <i>tumefaciens</i> were observed at a concentration of above 400 mg/l in the culture medium. The results suggested that kanamycin could be used as both counter-selection of <i>Agrobacterium</i> and selectable markers via <i>Agrobacterium</i>-mediated transformation in <i>P</i>. <i>yezoensis</i>, and that cefotaxime could be used as counter-selection of <i>Agrobacterium</i> only.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Effects of cefotaxime and kanamycin on thallus proliferation and differentiation in <i>Porphyra yezoensis</i> and their inhibition on <i>Agrobacterium tumefaciens</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-24 01:31:50.513770","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Agrobacterium tumefaciens; antibiotics; genetic transformation; Porphyrayezoensis; thallium","OtherDescriptors":null,"Notes":null,"AnaPub":2010,"MonPub":null,"DateUpdate":"2021-11-25","DateCreate":"2010-02-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000274344900008","VABBcode":null,"OpenAcc":0,"DOI":"10.1080/17451000902933017"},"refs":null,"anarec":{"AnaID":143657,"PubliDate":2010,"Pagination":"100-105","XtraPublOfAnaID":null,"ISBN":null,"Volume":"6","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":72882,"SerRR":"Marine Biology Research. 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