{"refrec":{"BRefID":144144,"RR":"Sakshaug, E.; Holm-Hansen, O. (1977). Chemical composition of Skeletonema costatum (Grev.) Cleve and Paplova (Monochrysis) lutheri (Droop) Green as a function of nitrate-, phosphate-, and iron-limited growth. J. Exp. Mar. Biol. Ecol. 29(1): 1-34. https://dx.doi.org/10.1016/0022-0981(77)90118-6","BEntID":137288,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". J. Exp. Mar. Biol. Ecol. 29(1): 1-34. https://dx.doi.org/10.1016/0022-0981(77)90118-6","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Sakshaug, E.; Holm-Hansen, O.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Sakshaug, E.; Holm-Hansen, O.","Englishabstract":"The variation in chemical composition of S. costatum and P. lutheri was studied in batch cultures with N-, P-, and Fe-deficient media under continuous light. In vivo fluorescence, chlorophyll a, ATP, cellular nitrogen, carbon, and phosphorus, and cell density were measured. The exponential growth rate was 2 . 9-3 . 5 doubl. Day-1 for Skeletonema and 1 . 2-1 . 9 doubl. Day-1 for Pavlova, with the lower rates pertaining to cultures in Fe-deficient media. In exponentially growing cultures nitrogen and carbon per cell increased with an increasing N/P ratio of the media, whereas chlorophyll a, ATP, and organic phosphorus per cell remained relatively constant. In Fe-deficient cells nitrogen and carbon per cell was high, as for cells in media with a high N/P ratio. The total variation in exponential cells was contained within a factor of three. Larger differences in chemical composition were found between exponentially growing cells and nutrient deficient cells. For all types of nutrient deficiency the chl/N and chl/C ratios decreased when cells went from the exponential phase to the starvation phase. The ratio between in vivo fluorescence and chlorophyll a increased 4-5 fold. The C/N ratio (atomic) of both species increased from 5-7 . 5 for exponentially growing cells up to 35 during N-deficiency and up to 13 during P-deficiency. For cells in Fe-deficient medium values were scattered in the 7-11 range. ATP and organic phosphorus followed each other closely and had values which were about 3 times higher in Skeletonema than in Pavlova when using any other parameter as a frame of reference for identical media and growth stages. In N-deficient media the N/ATP ratio was virtually the same for exponentially growing cells and starved cells. The C/ATP ratio reached high levels in nutrient deficient cells, with a maximum of 9000 for P-deficient cells of Pavlova. Both species had a minimum value of polyphosphates corresponding to about 10% of the total phosphorus. In P-rich media Pavlova had storage polyphosphates up to 70% of the total P. Storage polyphosphates were not observed in Skeletonema. With the set of factors used it is possible to identify growth stages and type of nutrient deficiency for a given culture. It is indicated that, with certain limitations, this approach may be applicable in field studies.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Chemical composition of Skeletonema costatum (Grev.) Cleve and Paplova (Monochrysis) lutheri (Droop) Green as a function of nitrate-, phosphate-, and iron-limited growth","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-20 01:31:51.182967","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1977,"MonPub":null,"DateUpdate":"2020-11-03","DateCreate":"2010-03-11","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1977EB76300001","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/0022-0981(77)90118-6"},"refs":null,"anarec":{"AnaID":144144,"PubliDate":1977,"Pagination":"1-34","XtraPublOfAnaID":null,"ISBN":null,"Volume":"29","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43229,"SerRR":"Journal of Experimental Marine Biology and Ecology. Elsevier: New York. ISSN 0022-0981; e-ISSN 1879-1697","StandardTitleSer":"Journal of Experimental Marine Biology and Ecology","ISSN":"0022-0981","AbbrevSer":"J. Exp. Mar. Biol. Ecol.","StandardTitleMon":null,"StartPage":1,"Pages":34,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"Trondheim","Email":null,"Line2":null,"EnvName":"Norway"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Sakshaug","Firstname":"Egil","Initials":"E.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":137288,"AutID":54887,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Holm-Hansen","Firstname":null,"Initials":"O.","Affiliation":null,"Discriminator":null,"CorporateFlag":1,"BEntID":137288,"AutID":90978,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":320992,"LibID":36,"BRefID":144144,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"320992","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"
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