{"refrec":{"BRefID":15096,"RR":"<b>Drouin, H.; de la Noüe, J.; Lavoie, M.</b> (1989). Activation of a nitrifying biofilter using a pH controller (abstract), <b><i>in</i></b>: De Pauw, N. <i>et al.</i> (Ed.) <i>Aquaculture: a biotechnology in progress: volume 1.</i> pp. 1069","BEntID":15096,"PublicFlag":1,"CheckedFlag":0,"wosflag":null,"vabbflag":null,"RefStringPartII":", <b><i>in</i></b>: De Pauw, N. <i>et al.</i> <i>Aquaculture: a biotechnology in progress: volume 1.</i> pp. 1069","DocTypID":17,"DocType":"Book chapters","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Drouin, H.; de la Noüe, J.; Lavoie, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Drouin, H.; de la Noüe, J.; Lavoie, M.","Englishabstract":"The activation of a biofilter is a slow process that generally requires between 4 and 8 weeks. The sole energy source of autotrophic nitrifying bacteria (oxidation of ammonia and nitrite) is a low-energy yielding process. The nitrifiers are thus slow growers. Our goal was to establish within the shortest possible time, a stable population of nitrifying bacteria, capable of performing upon connection to a full tank of fish. Trickling filters filled with polystyrene support (220m².m<sup>-3</sup>) were inoculated with an enriched culture prepared by incubation of sludge from a previous filter in <i>Nitrosomonas</i> medium (Sato ). After the exhaustion of the ammonia initially added, the filters were continuously injected with ammonia (NH<sub>4</sub>Cl), simulating a given fish load. A constant pH (8.5) was maintained by the automatic addition of sodium carbonate with a pH stat. The daily parameters followed were: NH<sub>4</sub>+, NO<sub>2</sub>-, NO<sub>3</sub>-, dissolved oxygen (DO). alkalinity, Na<sub>2</sub>CO<sub>3</sub> consumption, and hardness. Free and immobilized nitrifiers (<i>Nitrosomonas</i> sp.. <i>Nitrobacter</i> sp.). heterotrophic bacteria, and denitrifiers were enumerated. After 6 days, the NH<sub>4</sub>+ concentration decreased below 0. 7mgI1 with a maintenance average of 0.4:tO 18mgI1. It took 8 days for NOz- to decrease below 0.5 mg.l<sup>-1</sup> (average = 0.1:tO.13mg.l<sup>-1</sup>). The nitrate concentration reached 86.2mg.l<sup>-1</sup> Alkalinity (as CaCO<sub>3</sub>), hardness (as CaC0<sub>3</sub>), and dissolved oxygen (DO) were relatively stable with respective mean concentratioons of 170±34mg.l<sup>-1</sup>, 197±20mg.l<sup>-1</sup> and 8.3±0.3 mg.l<sup>-1</sup>. Approximately 10g of alkalinity (as CaC0<sub>3</sub>) was consumed for every g of ammonia (NH<sub>4</sub>+) injected. By using an inoculum enriched for nitrifying bacteria. and maintaining the pH at 8.5 with Na<sub>2</sub>C0<sub>3</sub> we succeeded in activating a nitrifying trickling-filter in approximately 8 days. This research was supported by FCAR-Equipe and CRSAQ grants.","AbstractOtherLang":null,"BibLvlCode":"AM","StandardTitle":"Activation of a nitrifying biofilter using a pH controller (abstract)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:01.897972","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1989,"MonPub":null,"DateUpdate":"2001-08-21","DateCreate":"2001-08-08","SecASFANote":null,"ConfID":null,"PeerRev":null,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":15096,"PubliDate":1989,"Pagination":"1069","XtraPublOfAnaID":null,"ISBN":"90-71625-03-6","Volume":"1","Issue":null,"BRefMon":14854,"BRefMonRR":"<b>De Pauw, N. <i>et al.</i></b> (1989). Aquaculture: a biotechnology in progress: volume 1. European Aquaculture Society: Bredene. 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