{"refrec":{"BRefID":16870,"RR":"<b>Hoffmann, R.J.</b> (1985). Thermal adaptation and the properties of phosphoglucose isomerase allozymes from a sea anemone, <b><i>in</i></b>: Gibbs, P.E. (Ed.) <i>Proceedings of the 19th European Marine Biology Symposium, Plymouth, Devon, UK, 16-21 September 1984.</i> pp. 505-514","BEntID":16870,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":", <b><i>in</i></b>: Gibbs, P.E. (Ed.) <i>Proceedings of the Nineteenth European Marine Biology Symposium, Plymouth, Devon, UK, 16-21 September 1984. European Marine Biology Symposia,</i> 19: pp. 505-514","DocTypID":17,"DocType":"Book chapters","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Hoffmann, R.J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Hoffmann, R.J.","Englishabstract":"Populations of the sea anemone <i>Metridium senile</i> from the northeast coast of North America have two alleles that code for the glycolytic enzyme phosphoglucose isomerase (E.C. 5.3.1.9). Allozymes produced by alternate homozygotes differ in their heat stabilities. The locus exhibits clinal variation, with the allele encoding the electrophoretically fast allozyme (<i>Pgi</i><sup>f</sup>) being nearly fixed in populations south of Cape Cod. The allele for the slow form (<i>Pgi</i><sup>8</sup>) increases in frequency as populations are sampled farther to the north, and its frequency reaches an observed maximum of approximately 0.5 in northern Maine and southern Canada, the limits of sampling to date. Paralleling this cline in allele frequency is a thermal gradient in the summertime mean temperature. These patterns suggest the hypothesis that the fast allozyme is adapted to warm temperatures and the slow allele to colder temperatures. Partial support for a thermal selection hypothesis comes from examination of the kinetics of partially purified preparations of the allozymes from the genotype. There are significant differences in activity between forms, with the allozyme from <i>Pgi</i><sup>ff</sup> homozygotes being about 2.5- to 3-fold higher in specific activity than that from <i>Pgi</i><sup>88</sup> homozygotes. Heterozygotes produce a mixture of enzymes with intermediate activity. In addition, there are significant differences in <i>K</i><sub>m</sub> for substrates in both reaction directions. In the gluconeogenic reaction direction, the fast form has slightly higher <i>K</i><sub>m</sub> of fructose- 6-phosphate than the slow form at all temperatures from 5-25°c. In the glycolytic direction, the ordering of <i>K</i><sub>m</sub> of glucose-6-phosphate is reversed, with the slow form exhibiting the higher values at all temperatures. <i>V</i><sub>max</sub>/ <i>K</i><sub>m</sub> ratios are also significantly different, with <i>Pgi</i><sup>ff</sup> homozygotes showing higher values at all tested temperatures. The greatest absolute difference in this ratio falls at 25°c, near the maximum temperature experienced in nature. These data suggest a biochemical reason for the predominance of the fast allozyme in warm waters resulting from its superior activity at high environmental temperatures, but they leave unexplained the reason for the rise in frequency of the slow form in colder waters.","AbstractOtherLang":null,"BibLvlCode":"AMS","StandardTitle":"Thermal adaptation and the properties of phosphoglucose isomerase allozymes from a sea anemone","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-07-08 01:30:39.408765","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1985,"MonPub":null,"DateUpdate":"2022-11-21","DateCreate":"2001-08-29","SecASFANote":null,"ConfID":null,"PeerRev":0,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":16870,"PubliDate":1985,"Pagination":"505-514","XtraPublOfAnaID":null,"ISBN":"0-521-30294-3","Volume":"19","Issue":null,"BRefMon":16823,"BRefMonRR":"<b>Gibbs, P.E. (Ed.)</b> (1985). Proceedings of the Nineteenth European Marine Biology Symposium, Plymouth, Devon, UK, 16-21 September 1984. <i>European Marine Biology Symposia</i>, 19. Cambridge University Press: Cambridge. ISBN 0-521-30294-3. 541 pp.","BRefXtra":null,"BRefXtraRR":null,"SerBRefID":20275,"SerRR":"European Marine Biology Symposia.","StandardTitleSer":"European Marine Biology Symposia","ISSN":null,"AbbrevSer":null,"StandardTitleMon":"Proceedings of the Nineteenth European Marine Biology Symposium, Plymouth, Devon, UK, 16-21 September 1984","StartPage":505,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"MS","SerNotes":null,"AutString":"Gibbs, P.E. 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