{"refrec":{"BRefID":195548,"RR":"<b>Tada, Y.; Fujikura, K.; Oguri, K.; Kitazato, H.; Tanabe, K.</b> (2010). In situ fluorochrome calcein marking of deep-sea molluscs using a new growth chamber. <i>Aquat. Ecol. 44(1)</i>: 217-222. <a href=\"http://dx.doi.org/10.1007/s10452-009-9290-3\" target=\"_blank\">http://dx.doi.org/10.1007/s10452-009-9290-3</a>","BEntID":188020,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Ecol. 44(1)</i>: 217-222. <a href=\"https://dx.doi.org/10.1007/s10452-009-9290-3\" target=\"_blank\">https://dx.doi.org/10.1007/s10452-009-9290-3</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tada, Y.; Fujikura, K.; Oguri, K.; Kitazato, H.; Tanabe, K.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tada, Y. <i>et al.</i>","Englishabstract":"A new instrument designed for in situ chemical marking experiments was developed and applied to the deep-sea seep clams Calyptogena soyoae and Calyptogena okutanii. Fluorochrome calcein was used for vital staining of four living clams kept in a specially designed in situ growth chamber that was placed on the seafloor at a cold-seep site off Hatsushima Island, Sagami Bay, central Japan. The shell margins of both C. soyoae and C. okutanii were clearly stained, forming a thin fluorescent band. 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