{"refrec":{"BRefID":198208,"RR":"<b>Verhaegen, Y.; Parmentier, K.; Swevers, L.; Rougé, P.; Soin, T.; De Coen, W.; Cooreman, K.; Smagghe, G.</b> (2010). The brown shrimp (<i>Crangon crangon</i> L.) ecdysteroid receptor complex: Cloning, structural modeling of the ligand-binding domain and functional expression in an EcR-deficient <i>Drosophila</i> cell line. <i>Gen. Comp. Endocrinol. 168(3)</i>: 415-423. <a href=\"https://dx.doi.org/10.1016/j.ygcen.2010.05.007\" target=\"_blank\">https://dx.doi.org/10.1016/j.ygcen.2010.05.007</a>","BEntID":190583,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Gen. Comp. Endocrinol. 168(3)</i>: 415-423. <a href=\"https://dx.doi.org/10.1016/j.ygcen.2010.05.007\" target=\"_blank\">https://dx.doi.org/10.1016/j.ygcen.2010.05.007</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Verhaegen, Y.; Parmentier, K.; Swevers, L.; Rougé, P.; Soin, T.; De Coen, W.; Cooreman, K.; Smagghe, G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Verhaegen, Y. <i>et al.</i>","Englishabstract":"cDNAs encoding ecdysteroid receptor (EcR) and retinoid X receptor (RXR) were cloned and sequenced from brown shrimp <i>Crangon crangon</i> (Crustacea: Decapoda), a common faunal species and commercially important in the North-West European coastal waters. A 3D model of the ligand-binding domain (LBD) of EcR was created and docking of ponasterone A (PonA) was simulated <i>in silico</i>. Finally, we report the transfection of expression plasmids for these receptors in the mutant <i>Drosophila</i> L57-3-11 cell line. Through an ecdysteroid responsive reporter assay we clearly prove the functionality of shrimp ecdysteroid receptor in the transfected L57-3-11 cell line. Our results indicate that the <i>Drosophila</i> L57-3-11 cell line and <i>in silico</i> LBD modeling can be used to study the function of crustacean ecdysteroid receptors and be applied to assess endocrine disrupting effects on non-target crustacean species.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The brown shrimp (<i>Crangon crangon</i> L.) ecdysteroid receptor complex: Cloning, structural modeling of the ligand-binding domain and functional expression in an EcR-deficient <i>Drosophila</i> cell line","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2010,"MonPub":null,"DateUpdate":"2019-06-25","DateCreate":"2010-09-02","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000281126700014","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.ygcen.2010.05.007"},"refs":null,"anarec":{"AnaID":198208,"PubliDate":2010,"Pagination":"415-423","XtraPublOfAnaID":null,"ISBN":null,"Volume":"168","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42729,"SerRR":"General and Comparative Endocrinology. 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