{"refrec":{"BRefID":198880,"RR":"<b>El-Barbary, M.I.</b> (2010). Some clinical, microbiological and molecular characteristics of <i>Aeromonas hydrophila</i> isolated from various naturally infected fishes. <i>Aquacult. Int. 18(5)</i>: 943-954. <a href=\"https://dx.doi.org/10.1007/s10499-009-9315-x\" target=\"_blank\">https://dx.doi.org/10.1007/s10499-009-9315-x</a>","BEntID":191199,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Aquacult. Int. 18(5)</i>: 943-954. <a href=\"https://dx.doi.org/10.1007/s10499-009-9315-x\" target=\"_blank\">https://dx.doi.org/10.1007/s10499-009-9315-x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":1,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"El-Barbary, M.I.","OrigTitleTranslFlag":0,"Authorstringtrunc":"El-Barbary, M.I.","Englishabstract":"Heat-stable cytotonic enterotoxin gene (<i>Ast)</i> was detected by polymerase chain reaction (PCR) of twenty isolates of <i>Aeromonas hydrophila</i> isolated from various naturally infected fishes collected from both fresh and brackish water. These fishes were Nile tilapia and meagre, mullet and sea bream, respectively. Antibiotic susceptibility, pathogenic characteristics of these isolates and histopathological alterations of liver from experimentally infected tilapia fish with <i>A. hydrophila</i> which contained <i>Ast</i> gene were investigated. PCR technique for the detection of <i>Ast</i> as specific gene for <i>A.</i> <i>hydrophila</i> genomes showed that 90% of tested <i>A. hydrophila</i> (18/20) contained <i>Ast</i> gene, which is specific for <i>A. hydrophila</i> <i>(SSU</i>).The in vitro susceptibility of 18 strains of <i>A. hydrophila</i> (SSU) to 9 antibiotics was evaluated. Oxytetracycline only was an effective antibiotic for all tested isolates. On contrast, all these isolates were resistant to amoxicillin, ampicillin and penicillin. Pathogenicity assay in this study proved that 33.3% of the tested <i>A. hydrophila</i> (6/18) were pathogenic for tilapia in vitro with various levels of virulence where 2/6 were classified as strongly virulent according to the severity of mortality rate. Microscopically, <i>A. hydrophila</i> toxins apparently cause irreparable systemic damage to liver which leads to death.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Some clinical, microbiological and molecular characteristics of <i>Aeromonas hydrophila</i> isolated from various naturally infected fishes","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-12 01:31:22.386734","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"A. hydrophila (SSU); Fish; Ast gene; PCR; Antibiotics; Histopathology;Liver","OtherDescriptors":null,"Notes":null,"AnaPub":2010,"MonPub":null,"DateUpdate":"2020-11-03","DateCreate":"2010-09-28","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000280087300021","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10499-009-9315-x"},"refs":null,"anarec":{"AnaID":198880,"PubliDate":2010,"Pagination":"943-954","XtraPublOfAnaID":null,"ISBN":null,"Volume":"18","Issue":"5","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":44242,"SerRR":"Aquaculture International. 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