{"refrec":{"BRefID":199074,"RR":"<b>Zhang, Y.; Henriet, J.-P.; Bursens, J.; Boon, N.</b> (2010). Stimulation of in vitro anaerobic oxidation of methane rate in a continuous high-pressure bioreactor. <i>Bioresour. Technol. 101(9)</i>: 3132-3138. <a href=\"http://dx.doi.org/10.1016/j.biortech.2009.11.103\" target=\"_blank\">dx.doi.org/10.1016/j.biortech.2009.11.103</a>","BEntID":191382,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Bioresour. Technol. 101(9)</i>: 3132-3138. <a href=\"https://dx.doi.org/10.1016/j.biortech.2009.11.103\" target=\"_blank\">https://dx.doi.org/10.1016/j.biortech.2009.11.103</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Zhang, Y.; Henriet, J.-P.; Bursens, J.; Boon, N.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Zhang, Y. <i>et al.</i>","Englishabstract":"Anaerobic oxidation of methane coupled to sulphate reduction (SR-AOM) prevents oceanic methane emissions and is considered as a major environmental process in the deep-sea sediments. To stimulate in vitro  SR-AOM activity, we designed a (continuous) high-pressure bioreactor system. Fed-batch mode incubations showed that the elevated methane pressure stimulated SR-AOM activity: when the methane pressure increased from 1 to 4.5 to 8 MPa, the initial SR-AOM activity increased from 3.46 to 8.64 to 9.22 µmol sulphide production/gdw/day; the apparent affinity (Km) for methane was 37 mM. However, in each fed-batch mode incubation, there was an inhibitory effect observed after 2 days, probably due to the accumulation of sulphide and the increase of pH. 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