{"refrec":{"BRefID":199085,"RR":"<b>Riou, V.; Bouillon, S.; Serrão Santos, R.; Dehairs, F.; Colaço, A.</b> (2010). Tracing carbon assimilation in endosymbiotic deep-sea hydrothermal vent mytilid fatty acids by <sup>13</sup>C-fingerprinting. <i>Biogeosciences 7(9)</i>: 2591-2600. <a href=\"http://dx.doi.org/10.5194/bg-7-2591-2010\" target=\"_blank\">dx.doi.org/10.5194/bg-7-2591-2010</a>","BEntID":191393,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Biogeosciences 7(9)</i>: 2591-2600. <a href=\"https://dx.doi.org/10.5194/bg-7-2591-2010\" target=\"_blank\">https://dx.doi.org/10.5194/bg-7-2591-2010</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Riou, V.; Bouillon, S.; Serrão Santos, R.; Dehairs, F.; Colaço, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Riou, V. <i>et al.</i>","Englishabstract":"Bathymodiolus azoricus</i> mussels thrive at Mid-Atlantic Ridge hydrothermal vents, where part oftheir energy requirements are met via an endosymbiotic association with chemolithotrophic and methanotrophic bacteria. In an effort to describe phenotypic characteristics of the two bacterial endosymbionts and to assesstheir ability to assimilate CO<sub>2</sub>, CH<sub>4</sub> and multi-carbon compounds, we performed experiments in aquaria using <sup>13</sup>C-labeled NaHCO<sub>3</sub> (in the presence of H<sub>2</sub>S), CH<sub>4</sub> or amino-acids and traced the incorporation of <sup>13</sup>C into total and phospholipid fatty acids (tFA and PLFA, respectively). 14:0; 15:0; 16:0; 16:1(<i>n</i> - 7)<i>c+t</i>; 18:1(<i>n</i> - 13)<i>c+t</i> and (<i>n</i> - 7)<i>c+t</i>; 20:1(<i>n</i> - 7); 20:2(<i>n</i> - 9,15); 18:3(<i>n</i> - 7) and (<i>n</i> - 5,10,13) PLFA were labeled in the presence of H<sup>13</sup>CO<sub>3</sub><sup>-</sup> (+H<sub>2</sub>S) and <sup>13</sup>CH<sub>4</sub>, while the 12:0 compound became labeled only in the presence ofH<sup>13</sup>CO<sub>3</sub><sup>-</sup> (+H<sub>2</sub>S). In contrast, the 17:0; 18:0; 16:1(<i>n</i> - 9); 16:1(<i>n</i> - 8) and (<i>n</i> - 6); 18:1(<i>n</i> - 8); and 18:2(<i>n</i> - 7) PLFA were only labeled in the presence of <sup>13</sup>CH<sub>4</sub>. Some of these symbiont-specific fatty acids also appeared to be labeled in mussel gill tFA when incubated with <sup>13</sup>C-enriched amino acids, and so were mussel-specific fatty acids such as 22:2(<i>n</i> - 7,15). Our results provide experimental evidence for the potential of specific fatty acid markers to distinguish between the two endosymbiotic bacteria, shedding new light on C<sub>1</sub> and multi-carbon compound metabolic pathways in <i>B. azoricus</i> and its symbionts.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Tracing carbon assimilation in endosymbiotic deep-sea hydrothermal vent mytilid fatty acids by <sup>13</sup>C-fingerprinting","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2010,"MonPub":null,"DateUpdate":"2014-10-09","DateCreate":"2010-10-05","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000282427800001","VABBcode":null,"OpenAcc":1,"DOI":"10.5194/bg-7-2591-2010"},"refs":null,"anarec":{"AnaID":199085,"PubliDate":2010,"Pagination":"2591-2600","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"9","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":68092,"SerRR":"<b>Gattuso, J.P.; Kesselmeier, J. (Ed.)</b> Biogeosciences. Copernicus Publications: Göttingen.  ISSN 1726-4170; e-ISSN 1726-4189","StandardTitleSer":"Biogeosciences","ISSN":"1726-4170","AbbrevSer":"Biogeosciences","StandardTitleMon":null,"StartPage":2591,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Riou","Firstname":"Virginie","Initials":"V.","Affiliation":"Department of Analytical and Environmental Chemistry, Vrije Universiteit Brussel","Discriminator":null,"CorporateFlag":0,"BEntID":191393,"AutID":125677,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"AMGC","InsFSN":"Vrije Universiteit Brussel; Faculteit Wetenschappen & Bio-ingenieurswetenschappen; Vakgroep Chemie; Analytical, Environmental and Geochemistry","ORCID":"0000-0002-6549-385X","PersID":23675,"InsID":40},{"AutName":"Bouillon","Firstname":"Steven","Initials":"S.","Affiliation":"Department of Analytical and Environmental Chemistry, Vrije Universiteit Brussel","Discriminator":null,"CorporateFlag":0,"BEntID":191393,"AutID":143928,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"AMGC","InsFSN":"Vrije Universiteit Brussel; 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