{"refrec":{"BRefID":199609,"RR":"<b>Nikapitiya, C.; Oh, C.; De Zoysa, M.; Whang, I.; Kang, D.-H.; Lee, S.-R.; Kim, S.-J.; Lee, J.</b> (2010). Characterization of beta-1,4-endoglucanase as a polysaccharide-degrading digestive enzyme from disk abalone, <i>Haliotis discus discus</i>. <i>Aquacult. Int. 18(6)</i>: 1061-1078. <a href=\"https://dx.doi.org/10.1007/s10499-010-9324-9\" target=\"_blank\">https://dx.doi.org/10.1007/s10499-010-9324-9</a>","BEntID":191902,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Aquacult. Int. 18(6)</i>: 1061-1078. <a href=\"https://dx.doi.org/10.1007/s10499-010-9324-9\" target=\"_blank\">https://dx.doi.org/10.1007/s10499-010-9324-9</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Nikapitiya, C.; Oh, C.; De Zoysa, M.; Whang, I.; Kang, D.-H.; Lee, S.-R.; Kim, S.-J.; Lee, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Nikapitiya, C. <i>et al.</i>","Englishabstract":"Gene coding for a putative endoglucanase was isolated and characterized from a disk abalone <i>Haliotis discus discus</i> cDNA library and was named as <i>hdEndgI</i>. The full length (1,908 bp) abalone cDNA contained a 1,824 bp open reading frame (ORF), encoding 608 amino acids (aa). The predicted molecular mass and isoelectric point were 65 kDa and 4.5, respectively. The C-terminal region showed conserved catalytic site regions, with the catalytic site residues responsible for the endoglucanase activity. RT–PCR results revealed greater <i>hdEndgI</i> mRNA expression in the hepatopancreas than in the digestive tract, suggesting the key site at which the enzyme is produced. <i>hdEndgI</i> mRNA expression decreased from baseline to the 8th week of starvation in the hepatopancreas; and once re-feeding was initiated, mRNA transcription increased to normal levels within 14 days. This suggests that gene transcription is not totally disturbed due to starvation-induced stress, showing the potential of <i>hdEndgI</i> as a molecular tool for identification of food availability. It further suggests that abalone can survive for 8 weeks without food. The purified <i>hdEndgI</i> recombinant fusion protein was shown to have in vitro activity against carboxymethylcellulose. The enzyme exhibits a broad range of pH stability, the optimal temperature and pH being 40°C and 4.5, respectively.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Characterization of beta-1,4-endoglucanase as a polysaccharide-degrading digestive enzyme from disk abalone, <i>Haliotis discus discus</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-20 01:32:16.147601","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Abalone; Beta-1,4-endoglucanase; Cellulase; mRNA expression; Starvation","OtherDescriptors":null,"Notes":null,"AnaPub":2010,"MonPub":null,"DateUpdate":"2020-11-03","DateCreate":"2010-10-26","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000282316700008","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10499-010-9324-9"},"refs":null,"anarec":{"AnaID":199609,"PubliDate":2010,"Pagination":"1061-1078","XtraPublOfAnaID":null,"ISBN":null,"Volume":"18","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":44242,"SerRR":"Aquaculture International. 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