{"refrec":{"BRefID":199610,"RR":"<b>Rigos, G.; Samartzis, A.; Henry, M.; Fountoulaki, E.; Cotou, E.; Sweetman, J.; Davies, S.; Nengas, I.</b> (2010). Effects of additive iron on growth, tissue distribution, haematology and immunology of gilthead sea bream, <i>Sparus aurata</i>. <i>Aquacult. Int. 18(6)</i>: 1093-1104. <a href=\"https://dx.doi.org/10.1007/s10499-010-9326-7\" target=\"_blank\">https://dx.doi.org/10.1007/s10499-010-9326-7</a>","BEntID":191903,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Aquacult. Int. 18(6)</i>: 1093-1104. <a href=\"https://dx.doi.org/10.1007/s10499-010-9326-7\" target=\"_blank\">https://dx.doi.org/10.1007/s10499-010-9326-7</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Rigos, G.; Samartzis, A.; Henry, M.; Fountoulaki, E.; Cotou, E.; Sweetman, J.; Davies, S.; Nengas, I.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Rigos, G. <i>et al.</i>","Englishabstract":"The comparative effects of iron-supplemented levels on growth, tissue distribution, haematology and immunology of gilthead sea bream, <i>Sparus aurata</i> (2 g) were investigated, using four organic (50, 100, 200, 300 mg ORG/kg diet) and one inorganic iron source (200 INOR mg/kg diet).  Fish were treated for 12 weeks with the experimental diets and maintained at a water temperature of 19–22°C. Growth (final weight and specific growth rate), tissue distribution (spleen, liver and muscle), haematological parameters (red blood cells, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration) and non-specific immune indexes (respiratory burst activity and antibacterial activity of serum) were analysed. No significant differences were found in growth and iron tissue distribution among the tested groups. Red blood cell counting was statistically higher in fish given 50 ORG, 100 ORG, 200 ORG and 200 INOR feeds. However, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration were not significantly affected by increasing dietary iron. Fish receiving the 100 ORG diet had the best performance with respect to the respiratory burst activity and significantly higher values for antibacterial activity of serum were obtained in fish fed with the 300 ORG diet. The present findings provided no clear evidence of the optimum iron concentration. 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