{"refrec":{"BRefID":199694,"RR":"<b>Hollants, J.; Leliaert, F.; De Clerck, O.; Willems, A.</b> (2010). How endo- is endo-? Surface sterilization of delicate samples: A <i>Bryopsis</i> (Bryopsidales, Chlorophyta) case study. <i>Symbiosis 51(1)</i>: 131-138. <a href=\"http://dx.doi.org/10.1007/s13199-010-0068-0\" target=\"_blank\">dx.doi.org/10.1007/s13199-010-0068-0</a>","BEntID":191987,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Symbiosis 51(1)</i>: 131-138. <a href=\"https://dx.doi.org/10.1007/s13199-010-0068-0\" target=\"_blank\">https://dx.doi.org/10.1007/s13199-010-0068-0</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Hollants, J.; Leliaert, F.; De Clerck, O.; Willems, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Hollants, J. <i>et al.</i>","Englishabstract":"In the search for endosymbiotic bacteria, elimination of ectosymbionts is a key point of attention. Commonly, the surface of the host itself or the symbiotic structures are sterilized with aggressive substances such as chlorine or mercury derivatives. Although these disinfectants are adequate to treat many species, they are not suitable for surface sterilization of delicate samples. In order to study the bacterial endosymbionts in the marine green alga <i>Bryopsis</i>, the host plant's cell wall was mechanically, chemically and enzymatically cleaned. Merely a chemical and enzymatic approach proved to be highly effective. <i>Bryopsis</i> thalli treated with cetyltrimethylammonium bromide (CTAB) lysis buffer, proteinase K and bactericidal cleanser Umonium Master showed no bacterial growth on agar plates or bacterial fluorescence when stained with a DNA fluorochrome. Moreover, the algal cells were intact after sterilization, suggesting endophytic DNA is still present within these algae. This new surface sterilization procedure opens the way to explore endosymbiotic microbial communities of other, even difficult to handle, samples.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"How endo- is endo-? Surface sterilization of delicate samples: A <i>Bryopsis</i> (Bryopsidales, Chlorophyta) case study","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Bryopsis ; Endosymbionts; Endosymbiotic bacteria; Marine green algae; Surface sterilization","OtherDescriptors":null,"Notes":null,"AnaPub":2010,"MonPub":null,"DateUpdate":"2014-10-09","DateCreate":"2010-10-28","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000280810400011","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s13199-010-0068-0"},"refs":null,"anarec":{"AnaID":199694,"PubliDate":2010,"Pagination":"131-138","XtraPublOfAnaID":null,"ISBN":null,"Volume":"51","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43941,"SerRR":"Symbiosis. Springer: Philadelphia, Pa..  ISSN 0334-5114; e-ISSN 1878-7665","StandardTitleSer":"Symbiosis","ISSN":"0334-5114","AbbrevSer":"Symbiosis","StandardTitleMon":null,"StartPage":131,"Pages":8,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Hollants","Firstname":"Joke","Initials":"J.","Affiliation":"Laboratory of Microbiology, Biochemistry and Microbiology Department (WE10), Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":191987,"AutID":133572,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biochemie en Microbiologie; Laboratorium voor Microbiologie","ORCID":null,"PersID":19292,"InsID":1605},{"AutName":"Leliaert","Firstname":"Frederik","Initials":"F.","Affiliation":"Phycology Research Group and Center for Molecular Phylogenetics and Evolution, Biology Department, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":191987,"AutID":133574,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biologie; Onderzoeksgroep Fycologie","ORCID":"0000-0002-4627-7318","PersID":227,"InsID":651},{"AutName":"De Clerck","Firstname":"Olivier","Initials":"O.","Affiliation":"Phycology Research Group and Center for Molecular Phylogenetics and Evolution, Biology Department, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":191987,"AutID":133575,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biologie; Onderzoeksgroep Fycologie","ORCID":"0000-0002-3699-8402","PersID":2518,"InsID":651},{"AutName":"Willems","Firstname":"Anne","Initials":"A.","Affiliation":"Laboratory of Microbiology, Biochemistry and Microbiology Department (WE10), Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":191987,"AutID":133576,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biochemie en Microbiologie; Laboratorium voor Microbiologie","ORCID":"0000-0002-8421-2881","PersID":10817,"InsID":1605}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":215665,"LibID":36,"BRefID":199694,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"215665","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open Marien Archief","URL":null,"ClassifID":235,"Classification":"Open Marine Archive","ReqLink":null,"ClassifTypID":5,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":0,"InternalReq":0,"LoggedInReq":0,"Disclaimer":"Disclaimer_OMA","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Springer","City":"Philadelphia, Pa."}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Bacteria"},{"TaxTerm":"Bryopsis"},{"TaxTerm":"Chlorophyta"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":191987,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":191987,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1007/s13199-010-0068-0","externalID":"10.1007/s13199-010-0068-0","URLTypeCode":"DOI","URLID":19140,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Bacteria","AphiaID":6,"TaxtID":11315},{"TaxTerm":"Bryopsis","AphiaID":143812,"TaxtID":39195},{"TaxTerm":"Chlorophyta [green algae]","AphiaID":801,"TaxtID":15129}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Bacteria","DutchTerm":null,"AphiaID":6,"TaxtID":11315},{"TaxTerm":"Bryopsis","DutchTerm":null,"AphiaID":143812,"TaxtID":39195},{"TaxTerm":"Chlorophyta","DutchTerm":"Groenwieren","AphiaID":801,"TaxtID":15129}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Haspeslagh, Jan, J.","newSesDate":{"date":"2010-10-28 09:07:07.540000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Lyssens, Liesbeth, L.","updSesDate":{"date":"2014-10-09 20:45:17.310000","timezone_type":3,"timezone":"Europe/Brussels"}}}