{"refrec":{"BRefID":20507,"RR":"<b>Cho, E.S.; Park, J.G.; Oh, B.C.; Cho, Y.C.</b> (2001). The application of species specific DNA-targeted probes and fluorescently tagged lectin to differentiate several species of <i>Pseudo-nitzschia</i> (Bacillariophyceae) in Chinhae Bay, Korea. <i>Sci. Mar. (Barc.) 65(3)</i>: 207-214","BEntID":20507,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Sci. Mar. (Barc.) 65(3)</i>: 207-214","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Cho, E.S.; Park, J.G.; Oh, B.C.; Cho, Y.C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Cho, E.S. <i>et al.</i>","Englishabstract":"We applied molecular methods using a DNA probe and fluorescently tagged lectins to discriminate toxic <i>Pseudo-nitzschia multiseries</i> from the Chinhae Bay for a Korean harmfulalgae monitoring program. From the binding activity of lectins, <i>P. multistriata, P. subfraudulenta, P. pungens, P. multiseries</i> and <i>P. cuspidata</i> bound ConA, whereas <i>P. Sub-pacifica</i> and <i>P. delicatissima</i> did not. Ribosomal RNA-targeted oligonucleotide probes (muD1, puD1 and deD1) specifically reacted to <i>P. multiseries, P. pungens</i> and <i>P. delicatissima</i>, respectively, whereas auD1, frD1 and amD1 probes did not bind to <i>P. multiseries, P. pungens, P. cuspidata, P. multistriata, P. subpacifica, P. subfraudulenta</i> and <i>P. delicatissima</i>. In particular, fluorescent FITC-conjugated WGA specifically bound to <i>P. multiseries</i> but not to <i>P. pungens</i>, indicating that this is a desirable method for their rapid and easy discrimination. In addition, we tested a species-specific oligonucleotide DNA probe (muD1) using the whole cell hybridization filter tube system, and the WGA lectin probe to discriminate <i>P. multiseries</i> in the field, The oligonucleotide probe and fluorescent WGA bound specifically to <i>P. multiseries</i> and these labelled cells were correlated to label cell abundance. These results imply that DNA and lectin probes are apporopriate tools for counting <i>P. multiseries</i> and distinguishing morphologically similar Pseudo-nitzschia species in natural samples, therefore, these methods axe especially pertinent since rapid separation and quantitative estimation of cell abundance of <i>P. multiseries</i> are now important for a routine harmful algae monitoring program in Korean waters.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The application of species specific DNA-targeted probes and fluorescently tagged lectin to differentiate several species of <i>Pseudo-nitzschia</i> (Bacillariophyceae) in Chinhae Bay, Korea","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":"Pseudo-nitzschia, differentiation, DNA probes, FITC-conjugated lectins\r\nDOMOIC ACID, IDENTIFICATION, PUNGENS","Notes":null,"AnaPub":2001,"MonPub":null,"DateUpdate":"2001-11-22","DateCreate":"2001-11-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000171361200005","VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":20507,"PubliDate":2001,"Pagination":"207-214","XtraPublOfAnaID":null,"ISBN":null,"Volume":"65","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43791,"SerRR":"Scientia Marina (Barcelona). Consejo Superior de Investigaciones Científicas. Institut de Ciènces del Mar: Barcelona.  ISSN 0214-8358; e-ISSN 1886-8134","StandardTitleSer":"Scientia Marina (Barcelona)","ISSN":"0214-8358","AbbrevSer":"Sci. Mar. 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