{"refrec":{"BRefID":205485,"RR":"<b>Mardhiah, U.</b> (2010). Molecular diversity in a hydrogen-consuming enrichment culture from the South Pacific gyre. MSc Thesis. Max Planck Institute for Marine Microbiology/Erasmus Mundus Master of Science in Marine Biodiversity and Conservation (EMBC): Bremen.  47 pp.","BEntID":197615,"PublicFlag":1,"CheckedFlag":0,"wosflag":null,"vabbflag":null,"RefStringPartII":". MSc Thesis. Max Planck Institute for Marine Microbiology/Erasmus Mundus Master of Science in Marine Biodiversity and Conservation (EMBC): Bremen.  47 pp.","DocTypID":5,"DocType":"Book/Monograph","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Mardhiah, U.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Mardhiah, U.","Englishabstract":"The South Pasific Gyre is known to be the most oligotrophic water body on earth. Due to a low a very low amount of organic carbon sediments and thus oxygen penetrates deeply into the sediment. Hydrogen produced through natural radiation and radiolysis of water was hypothesized to be a major source of energy for sediment microorganisms. During the Knox-02RR cruise in 2007, the first centimetres of this sediment were sampled and oxic slurries were enriched with an over pressure of H<sub>2</sub>/CO<sub>2</sub> (80:20) for 22 months at 4°C. Gas was consumed after this incubation suggesting the activity of <i>Knallgas</i> microorganisms. The microbial community was investigated with a 16S rRNA gene libraries and by T-RFLP. Analysis of 16S rRNA gene showed that only the genus <i>Halomonas</i> was found in the enrichment. This was confirmed by T-RFLP using the restriction enzyme EcoRI, HhaI and SacII. Isolation attempts yielded strains that affiliated to <i>Halomonas</i>. Degenerated primers for a hydrogen subunit did not enable the amplification of a hydrogenase gene. 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