The aim of this survey is to characterize the communities in different layers of the mud---column. Community DNA from different layers is extracted and a clone library is made for identification. These results are compared with identified isolates from the same sample grown on agar plate. The clone library presumes a wide diversity in which the aerobically cultivable isolates represent only a small fraction of the community. All clone libraries are dominated by novel uncultured species, in which uncultured Gamma--- and Deltaproteabacteriatake an important place. The different depth samples showed no clear shift in microbial community. But more clones in each library are needed to confirm these findings. Isolates on agar plate show an abundant presence of Bacillus sp. within the phylum Firmicutes. Also Proteobacteria and Bacteroidetes are present. EPS producing species will be identified (under anaerobic and aerobic conditions), separated and cultivated in the future. The bio---films will be extracted (after production in bio---reactors) and used for further rheology research on the harbor sediments.","AbstractOtherLang":"Bijzonder weinig is geweten over micro organismen in relatief onvervuild maritiem slib. Desondanks, wordt er aangenomen dat deze organismen van invloed zijn op de rheologische eigenschappen van de nautische bodem. Een duidelijk inzicht in deze microbiele gemeenschappen zou een belangrijke stap kunnen betekenen in het rheologisch onderzoek van maritiem slib, en kan een aanzienlijke besparing van de baggerkost met zich mee brengen.
Het doel van deze studie is de karakterisatie van microbiele gemeenschappen in de verschillende lagen van de slibkolom. Gemeenschaps---DNA van de verschillende lagen is geextraheerd en een clone library is aangelegd voor identificatie. Deze resultaten kunnen dan vergeleken worden met op agar plaat aeroob gekweekte isolaten afkomstig van dezelfde stalen. De clone library doet een zeer brede microbiele diversiteit vermoeden waarin de plaat--- gekweekte isolaten slecht een kleine fractie vertegenwoordigen van de gehele gemeenschap. Alle clone libraries worden gedomineerd door oncultiveerbare species waaronder een aanzienlijk deel Gamma--- en Deltaproteabacteria. De verschillende dieptestalen tonen geen microbiele shift van de gemeenschap aan. Maar meer clones per library zijn nodig om deze bevindingen te bevestigen. Isolaten op de agar platen tonen een dominante aanwezigheid van Bacillus sp. aan. Naast de phylum Firmicutes zijn ook Proteobacteria en Bacteroidetes aanwezig. In de toekomst zullen vooral EPSproducerende species geidentificeerd (onder aerobe en anaerobe condities), geisoleerd en gekweekt worden. De biofilms worden dan na productie in bioreactoren gescheiden en gebruikt voor verder reologisch onderzoek op het haven sediment.","BibLvlCode":"M","StandardTitle":"Characterization of microbial communities in marine mud and related biofilm production; a key step in mud rheology research","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 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