Document of bibliographic reference 207884

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Radioactive contamination of the marine environment: Uptake and distribution of ³H in Dunaliella bioculata

Abstract

The marine flagellate Dunaliella bioculata, which is easily cultivated under laboratory conditions, is a suitable organism for assessing the importance of the radioactive contamination by3H bound to organic molecules. We have studied the uptake of the following tritiated precursors: thymidine-methyl-3H, adenine-2-3H, uridine-5-3H, l-leucine-4-3H, glycine-2-3H, l-arginine-3.4-3H, 1-aspartic acid-2. 3-3H, 1-phenylalanine-2.3-3H, D-glucose-2-3H and D-glucose-6-3H. Under the experimental conditions (2000 lux; incubation time 30 min), all tritiated molecules are taken up by D. bioculata. Their intracellular concentration may reach that of the external medium. However, leucine and adenine accumulate in the algae: their respective concentrations are 10 and 100 times higher than in the culture medium. The molecular distribution of3H has been studied by various biochemical techniques and by sieve chromatography on sepharose 4B. It has been found that more l-leucine-4-3H is incorporated into acid and acetone soluble substances than into proteins. Adenine-2-3H is mainly incorporated into macromolecules of biological significance (RNA, DNA). CsCl gradient centrifugation has shown that the total DNA of Dunaliella is constituted by a major (p{variant}=1.707 g/cm3) and by a minor (p{variant}=1.693 g/cm3) component.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:A1980KS70700014

Bibliographic citation

Strack, S.; Bonotto, S.; Kirchmann, R. (1980). Radioactive contamination of the marine environment: Uptake and distribution of ³H in Dunaliella bioculata. Helgol. Meeresunters. 33(1-4): 153-163. https://dx.doi.org/10.1007/BF02414743

Topic

Marine

Is peer reviewed

true