{"refrec":{"BRefID":210852,"RR":"<b>Becker, P.; Gillan, D. C.; Eeckhaut, I.</b> (2007). Microbiological study of the body wall lesions of the echinoid <i>Tripneustes gratilla</i>. <i>Dis. Aquat. Org. 77(1)</i>: 73-82. <a href=\"http://dx.doi.org/10.3354/dao01821\" target=\"_blank\">dx.doi.org/10.3354/dao01821</a>","BEntID":202774,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Dis. Aquat. Org. 77(1)</i>: 73-82. <a href=\"https://dx.doi.org/10.3354/dao01821\" target=\"_blank\">https://dx.doi.org/10.3354/dao01821</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Becker, P.; Gillan, D. C.; Eeckhaut, I.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Becker, P. <i>et al.</i>","Englishabstract":"The microbiota of the body wall lesions of the echinoid <i>Tripneustes gratilla</i>, initiated by the grazing action of the parasitic gastropod <i>Vexilla vexillum</i>, was investigated with a pluridisciplinary approach. Parasitised sea urchins showed body wall lesions strongly infected by bacteria that progressed through the test and reached the coelomic cavity after ca. 1 mo. We report here on the bacterial community observed in lesions of echinoids collected in situ and on the bacteria that successively appeared during laboratory experiments. Two <i>Alphaproteobacteria</i>, a CFB (<i>Cytophaga-Flavobacterium-Bacteroides</i>) bacterium, 3 <i>Vibrio</i> species and <i>Exiguobacterium aestuarii</i> were identified in the field-collected lesions by 16S rDNA sequencing. The last 4 bacteria were cultured and each induced the disease when inoculated on scalpel-made wounds, with 100 % of the individuals infected within 2 d. Scalpel-induced scarifications tended to heal within 3 wk, while gastropod-induced lesions evolved into disease, suggesting a role of <i>Vexilla vexillum</i> in the development of the infection. Denaturing gradient gel electrophoresis (DGGE) and sequencing suggest that (1) bacteria associated with healthy integument were not present in the lesions and were thus not responsible for their infection, (2) <i>Alphaproteobacteria</i> with close phylogenetic affiliation with other bacteria involved in several diseases affecting marine invertebrates were present, and (3) these <i>Alphaproteobacteria</i> were present from the beginning of the infection and appeared earlier in the infection than other bacteria such as CFB bacteria.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Microbiological study of the body wall lesions of the echinoid <i>Tripneustes gratilla</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"echinoderm; DGGE; bacteria; infection; lesion; gastropod","OtherDescriptors":null,"Notes":null,"AnaPub":2007,"MonPub":null,"DateUpdate":"2017-02-07","DateCreate":"2011-12-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000249482300008","VABBcode":null,"OpenAcc":1,"DOI":"10.3354/dao01821"},"refs":null,"anarec":{"AnaID":210852,"PubliDate":2007,"Pagination":"73-82","XtraPublOfAnaID":null,"ISBN":null,"Volume":"77","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42792,"SerRR":"Diseases of Aquatic Organisms. 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