{"refrec":{"BRefID":210908,"RR":"<b>Baruah, K.; Ranjan, J.; Sorgeloos, P.; MacRae, T.H.; Bossier, P.</b> (2011). Priming the prophenoloxidase system of <i>Artemia franciscana</i> by heat shock proteins protects against <i>Vibrio campbellii</i> challenge. <i>Fish Shellfish Immunol. 31(1)</i>: 134-141. <a href=\"http://dx.doi.org/10.1016/j.fsi.2011.04.008\" target=\"_blank\">dx.doi.org/10.1016/j.fsi.2011.04.008</a>","BEntID":202830,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Fish Shellfish Immunol. 31(1)</i>: 134-141. <a href=\"https://dx.doi.org/10.1016/j.fsi.2011.04.008\" target=\"_blank\">https://dx.doi.org/10.1016/j.fsi.2011.04.008</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Baruah, K.; Ranjan, J.; Sorgeloos, P.; MacRae, T.H.; Bossier, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Baruah, K. <i>et al.</i>","Englishabstract":"Like other invertebrates, the brine shrimp <i>Artemia franciscana</i> relies solely on innate immunity, which by definition lacks adaptive characteristics, to combat against invading pathogens. One of the innate mechanisms is melanisation of bacteria mediated by the activation of the prophenoloxidase (proPO) system. The 70 kDa heat shock proteins (Hsp70) derived from either prokaryote (<i>Escherichia coli</i>) or eukaryote (<i>Artemia</i>), well conserved and immune-dominant molecules, protect <i>Artemia</i> against <i>Vibrio campbellii</i>. However, the molecular mechanisms by which these proteins protect <i>Artemia</i> against <i>Vibrio campbellii</i> infection are unknown. Here we demonstrated that feeding gnotobiotically grown <i>Artemia</i> with either <i>Artemia</i> Hsp70 or the <i>E. coli</i> Hsp70 equivalent DnaK, each overproduced in <i>E. coli</i>, followed by <i>V. campbellii</i> challenge enhanced the proPO system, at both mRNA and protein activity levels. Additionally, the <i>Artemia</i> fed with these proteins survived well in a <i>Vibrio</i> challenge assay. These results indicated that Hsp70s derived from either prokaryotic or eukaryotic sources generate protective immunity in the crustacean <i>Artemia</i> against <i>V. campbellii</i> infection by priming the proPO system. This is apparently the first in vivo report on priming activity of Hsp70 in an invertebrate.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Priming the prophenoloxidase system of <i>Artemia franciscana</i> by heat shock proteins protects against <i>Vibrio campbellii</i> challenge","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Heat shock protein (Hsp) 70; Phenoloxidase gene; Priming; Artemia","OtherDescriptors":null,"Notes":null,"AnaPub":2011,"MonPub":null,"DateUpdate":"2015-11-05","DateCreate":"2011-12-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000292407900016","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.fsi.2011.04.008"},"refs":null,"anarec":{"AnaID":210908,"PubliDate":2011,"Pagination":"134-141","XtraPublOfAnaID":null,"ISBN":null,"Volume":"31","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45027,"SerRR":"Fish & Shellfish Immunology. Academic Press: London; New York.  ISSN 1050-4648; e-ISSN 1095-9947","StandardTitleSer":"Fish & Shellfish Immunology","ISSN":"1050-4648","AbbrevSer":"Fish Shellfish Immunol.","StandardTitleMon":null,"StartPage":134,"Pages":8,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Baruah","Firstname":"Kartik","Initials":"K.","Affiliation":"Univ Ghent, Fac Biosci Engn, Lab Aquaculture, B-9000 Ghent, Belgium. ;   Univ Ghent, Artemia Reference Ctr, B-9000 Ghent, Belgium.","Discriminator":null,"CorporateFlag":0,"BEntID":202830,"AutID":139219,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":null,"PersID":16369,"InsID":22},{"AutName":"Ranjan","Firstname":"Jayant","Initials":"J.","Affiliation":"Univ Ghent, Fac Biosci Engn, Lab Aquaculture, B-9000 Ghent, Belgium. ; 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