{"refrec":{"BRefID":211044,"RR":"<b>Rojas-Garcia, C.R.; Sorgeloos, P.; Bossier, P.</b> (2009). Phenoloxidase and trypsin in germ-free larvae of <i>Artemia</i> fed with cooked unicellular diets: Examining the alimentary and protective effects of putative beneficial bacterium, yeast and microalgae against vibriosis. <i>J. Exp. Mar. Biol. Ecol. 381(2)</i>: 90-97. <a href=\"http://dx.doi.org/10.1016/j.jembe.2009.08.001\" target=\"_blank\">dx.doi.org/10.1016/j.jembe.2009.08.001</a>","BEntID":202966,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>J. Exp. Mar. Biol. Ecol. 381(2)</i>: 90-97. <a href=\"https://dx.doi.org/10.1016/j.jembe.2009.08.001\" target=\"_blank\">https://dx.doi.org/10.1016/j.jembe.2009.08.001</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Rojas-Garcia, C.R.; Sorgeloos, P.; Bossier, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Rojas-Garcia, C.R. <i>et al.</i>","Englishabstract":"Three putative beneficial unicellular organisms, the marine bacterium <i>Roseobacter</i> sp., the yeast <i>Saccharomyces cerevisiae</i> mnn9 strain (SC-mnn9) and the microalga <i>Tetraselmis suecica</i> were cooked and offered separately as diets to developing germ-free (GF) <i>Artemia</i> larvae, in order to analyze their alimentary and protective effects. GF <i>Artemia</i> larvae were able to grow with cooked <i>Tetraselmis</i> and SC-mnn9 but failed with cooked <i>Roseobacter</i>. in spite of its high dietary quality, <i>Tetraselmis</i> failed to provide protection against <i>Vibrio proteolyticus</i> infection, while <i>Roseobacter</i> failed as food as well to provide protection. Cooked SC-mnn9 appeared to possess both values, dietary for growth and protective against <i>Vibrio</i> infection. GF <i>Artemia</i> larvae were apparently rapid adapted to dietary swaps; from yeast to algal and from algal to yeast. While the diets swap from algal or yeast, to bacterial diet appeared to be detrimental. Phenoloxidase-L (PO-L) and trypsin-L were used as biochemical indicators of defense and digestive functions, respectively. Developmental trypsin-L patterns were similar when fed on yeast and microalgae diets, suggesting a good digestive adaptation to plant or fungal substrates at early stages. On the contrary, diets swap or <i>Vibrio</i> infection affected PO-L and trypsin-L suggesting a sort of 'alteration' of digestive and defense functions.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Phenoloxidase and trypsin in germ-free larvae of <i>Artemia</i> fed with cooked unicellular diets: Examining the alimentary and protective effects of putative beneficial bacterium, yeast and microalgae against vibriosis","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Artemia; Feeding; Innate immunity; Phenoloxidase; Trypsin; Vibrio; Yeast","OtherDescriptors":null,"Notes":null,"AnaPub":2009,"MonPub":null,"DateUpdate":"2015-11-05","DateCreate":"2011-12-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000272263500004","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.jembe.2009.08.001"},"refs":null,"anarec":{"AnaID":211044,"PubliDate":2009,"Pagination":"90-97","XtraPublOfAnaID":null,"ISBN":null,"Volume":"381","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43229,"SerRR":"Journal of Experimental Marine Biology and Ecology. 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