{"refrec":{"BRefID":211348,"RR":"<b>Lebaron, P.; Henry, A.; Lepeuple, A.S.; Pena, G.; Servais, P.</b> (2005). An operational method for the real-time monitoring of <i>E. coli</i> numbers in bathing waters. <i>Mar. Pollut. Bull. 50(6)</i>: 652-659. <a href=\"http://dx.doi.org/10.1016/j.marpolbul.2005.01.016\" target=\"_blank\">dx.doi.org/10.1016/j.marpolbul.2005.01.016</a>","BEntID":203270,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Mar. Pollut. Bull. 50(6)</i>: 652-659. <a href=\"https://dx.doi.org/10.1016/j.marpolbul.2005.01.016\" target=\"_blank\">https://dx.doi.org/10.1016/j.marpolbul.2005.01.016</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lebaron, P.; Henry, A.; Lepeuple, A.S.; Pena, G.; Servais, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lebaron, P. <i>et al.</i>","Englishabstract":"The aim of this study was to investigate the potential application of the ß-d-glucuronidase (GLUase) activity measurement for the routine detection and quantification of <i>E. coli</i> in marine bathing waters. GLUase activity was measured as the rate of hydrolysis of 4-methylumbelliferyl-ß-d-glucuronide. Culturable <i>E. coli</i> were quantified by the most probable number (MPN) microplate method. Both methods were applied to a large set of seawater samples. Significant correlation was found between the log of GLUase activity and the log of culturable <i>E. coli</i>. The mean coefficient of variation (CV) of the GLUase activity was less than 15% at concentrations around the current standards of International regulations whereas the CV of the microplate method was around 30%. When samples were stored at 4 °C and 20 °C, the mean CV of the GLUase activity remained below 15% up to 6 hours after sample collection whereas the range of variation of the microplate method varied between 10 and 50%. We concluded that the GLUase activity is an operational, reproducible, simple, very rapid and low cost method for the real-time enumeration of <i>E. coli</i> in bathing waters and should be preferred to the microplate method. The GLUase activity method should be routinely applied to the rapid enumeration of <i>E. coli</i> in recreational waters and recommendations for its application were suggested to water quality managers.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"An operational method for the real-time monitoring of <i>E. coli</i> numbers in bathing waters","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:01.897972","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"E. coli; bathing waters; real-time monitoring","OtherDescriptors":null,"Notes":null,"AnaPub":2005,"MonPub":null,"DateUpdate":"2015-11-05","DateCreate":"2011-12-22","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000230006400016","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.marpolbul.2005.01.016"},"refs":null,"anarec":{"AnaID":211348,"PubliDate":2005,"Pagination":"652-659","XtraPublOfAnaID":null,"ISBN":null,"Volume":"50","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43520,"SerRR":"Marine Pollution Bulletin. 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