{"refrec":{"BRefID":216575,"RR":"<b>Armani, A.; Castigliego, L.; Tinacci, L.; Gandini, G.; Gianfaldoni, D.; Guidi, A.</b> (2012). A rapid PCR–RFLP method for the identification of <i>Lophius</i> species. <i>Eur. Food Res. Technol. 235(2)</i>: 253-263. <a href=\"https://dx.doi.org/10.1007/s00217-012-1754-3\" target=\"_blank\">https://dx.doi.org/10.1007/s00217-012-1754-3</a>","BEntID":208324,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Eur. Food Res. Technol. 235(2)</i>: 253-263. <a href=\"https://dx.doi.org/10.1007/s00217-012-1754-3\" target=\"_blank\">https://dx.doi.org/10.1007/s00217-012-1754-3</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Armani, A.; Castigliego, L.; Tinacci, L.; Gandini, G.; Gianfaldoni, D.; Guidi, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Armani, A. <i>et al.</i>","Englishabstract":"The seven Anglerfish species, which belong to the genus <i>Lophius</i>, have a different value on the market, worldwide. If whole fishes can be identified by their morphological characteristics, they become indistinguishable when prepared or processed. In this study, a rapid method based on polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) was developed for the authentication of the seven <i>Lophius</i> species, using a cytochrome b gene fragment of 566 bp. After a genus-specific PCR, a fast digestion with the restriction enzyme <i>BfaI</i>, followed by agarose gel electrophoresis, allowed a clear species identification by producing specific restriction patterns. The total time required was as low as 6 h, DNA extraction included. The method was then used to analyse 48 commercial samples, whose phylogenetic analysis confirmed the PCR–RFLP response at 100 %. Results showed that mislabelling occurs on the market regardless the kind of processing.         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