{"refrec":{"BRefID":217424,"RR":"<b>Ver Eecke, H.C.; Butterfield, D.A.; Huber, J.A.; Lilley, M.D.; Olson, E.J.; Roe, K.K.; Evans, L.J.; Merkel, A.Y.; Cantin, H.V.; Holden, J.F.</b> (2012). Hydrogen-limited growth of hyperthermophilic methanogens at deep-sea hydrothermal vents. <i>Proc. Natl. Acad. Sci. U.S.A. 109(34)</i>: 13674-13679. <a href=\"http://dx.doi.org/10.1073/pnas.1206632109\" target=\"_blank\">dx.doi.org/10.1073/pnas.1206632109</a>","BEntID":209173,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Proc. Natl. Acad. Sci. U.S.A. 109(34)</i>: 13674-13679. <a href=\"https://dx.doi.org/10.1073/pnas.1206632109\" target=\"_blank\">https://dx.doi.org/10.1073/pnas.1206632109</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ver Eecke, H.C.; Butterfield, D.A.; Huber, J.A.; Lilley, M.D.; Olson, E.J.; Roe, K.K.; Evans, L.J.; Merkel, A.Y.; Cantin, H.V.; Holden, J.F.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ver Eecke, H.C. <i>et al.</i>","Englishabstract":"Microbial productivity at hydrothermal vents is among the highest found anywhere in the deep ocean, but constraints on microbial growth and metabolism at vents are lacking. We used a combination of cultivation, molecular, and geochemical tools to verify pure culture H<sub>2</sub> threshold measurements for hyperthermophilic methanogenesis in low-temperature hydrothermal fluids from Axial Volcano and Endeavour Segment in the northeastern Pacific Ocean. Two <i>Methanocaldococcus</i> strains from Axial and <i>Methanocaldococcus jannaschii</i> showed similar Monod growth kinetics when grown in a bioreactor at varying H<sub>2</sub> concentrations. Their H<sub>2</sub> half-saturation value was 66 µM, and growth ceased below 17–23 µM H<sub>2</sub>, 10-fold lower than previously predicted. By comparison, measured H<sub>2</sub> and CH<sub>4</sub> concentrations in fluids suggest that there was generally sufficient H<sub>2</sub> for <i>Methanocaldococcus</i> growth at Axial but not at Endeavour. Fluids from one vent at Axial (Marker 113) had anomalously high CH<sub>4</sub> concentrations and contained various thermal classes of methanogens based on cultivation and <i>mcrA</i>/<i>mrtA</i> analyses. At Endeavour, methanogens were largely undetectable in fluid samples based on cultivation and molecular screens, although abundances of hyperthermophilic heterotrophs were relatively high. Where present, <i>Methanocaldococcus</i> genes were the predominant <i>mcrA</i>/<i>mrtA</i> sequences recovered and comprised ~0.2–6% of the total archaeal community. Field and coculture data suggest that H<sub>2</sub> limitation may be partly ameliorated by H<sub>2</sub> syntrophy with hyperthermophilic heterotrophs. These data support our estimated H<sub>2</sub> threshold for hyperthermophilic methanogenesis at vents and highlight the need for coupled laboratory and field measurements to constrain microbial distribution and biogeochemical impacts in the deep sea.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Hydrogen-limited growth of hyperthermophilic methanogens at deep-sea hydrothermal vents","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2012,"MonPub":null,"DateUpdate":"2018-02-13","DateCreate":"2012-08-14","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000308085200049","VABBcode":null,"OpenAcc":1,"DOI":"10.1073/pnas.1206632109"},"refs":null,"anarec":{"AnaID":217424,"PubliDate":2012,"Pagination":"13674-13679","XtraPublOfAnaID":null,"ISBN":null,"Volume":"109","Issue":"34","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43690,"SerRR":"Proceedings of the National Academy of Sciences of the United States of America. 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