{"refrec":{"BRefID":218274,"RR":"<b>Veuger, B.; Middelburg, J.J.</b> (2007). Incorporation of nitrogen from amino acids and urea by benthic microbes: role of bacteria versus algae and coupled incorporation of carbon. <i>Aquat. Microb. Ecol. 48(1)</i>: 35-46. <a href=\"http://dx.doi.org/10.3354/ame048035\" target=\"_blank\">http://dx.doi.org/10.3354/ame048035</a>","BEntID":210017,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Microb. Ecol. 48(1)</i>: 35-46. <a href=\"http://dx.doi.org/10.3354/ame048035\" target=\"_blank\">http://dx.doi.org/10.3354/ame048035</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Veuger, B.; Middelburg, J.J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Veuger, B.; Middelburg, J.J.","Englishabstract":"We investigated the incorporation of nitrogen from amino acids and urea by the microbial community in intertidal surface sediment, focusing on the relative contributions of bacteria versus algae to total microbial nitrogen incorporation and the (un)coupled incorporation of carbon from these organic substrates. Dual-labeled (<SUP>15</SUP>N and <SUP>13</SUP>C) urea and an amino acid mixture were added to surface sediment from 2 intertidal mudflats in the Scheldt Estuary (The Netherlands), and <SUP>15</SUP>N and <SUP>13</SUP>C were subsequently traced into bulk sediment, total hydrolysable amino acids (THAAs) and the bacterial biomarker d-alanine (d-Ala) over a 24 h incubation period. All added <SUP>15</SUP>N from urea and the amino acids was incorporated into microbial biomass within 24 h, with relatively rapid incorporation of <SUP>15</SUP>N from the amino acids. The bacterial contribution to total microbial <SUP>15</SUP>N incorporation (derived from <SUP>15</SUP>N incorporation into d-Ala) was large during the first 1 to 2 h of incubation, but small to negligible after 24 h for both substrates, indicating that total <SUP>15</SUP>N incorporation was dominated by benthic microalgae (diatoms) that also dominated total microbial biomass in the sediment. Comparison of <SUP>15</SUP>N versus <SUP>13</SUP>C incorporation into total microbial biomass (THAAs) after 24 h showed strong preferential incorporation of urea-N over urea-C. Incorporation of nitrogen and carbon from the amino acids was partially uncoupled, indicating that a large fraction (=50%) of amino acid-N was taken up as NH<SUB>4</SUB><SUP>+</SUP> resulting from extracellular amino acid oxidation.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Incorporation of nitrogen from amino acids and urea by benthic microbes: role of bacteria versus algae and coupled incorporation of carbon","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-22 01:31:43.509315","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Nitrogen uptake; Urea; Amino acids; Bacteria; Benthic microalgae; Sediment; Hydrolysable amino acids; D-alanine","OtherDescriptors":null,"Notes":null,"AnaPub":2007,"MonPub":null,"DateUpdate":"2017-02-06","DateCreate":"2012-09-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000248017500005","VABBcode":null,"OpenAcc":1,"DOI":"10.3354/ame048035"},"refs":null,"anarec":{"AnaID":218274,"PubliDate":2007,"Pagination":"35-46","XtraPublOfAnaID":null,"ISBN":null,"Volume":"48","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42201,"SerRR":"Aquatic Microbial Ecology. 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