{"refrec":{"BRefID":219848,"RR":"<b>Silvestre, F.; Linares-Casenave, J.; Doroshov, S.I.; Kültz, D.</b> (2010). A proteomic analysis of green and white sturgeon larvae exposed to heat stress and selenium. <i>Sci. Total Environ. 408(16)</i>: 3176-3188. <a href=\"http://dx.doi.org/10.1016/j.scitotenv.2010.04.005\" target=\"_blank\">http://dx.doi.org/10.1016/j.scitotenv.2010.04.005</a>","BEntID":211582,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Sci. Total Environ. 408(16)</i>: 3176-3188. <a href=\"http://dx.doi.org/10.1016/j.scitotenv.2010.04.005\" target=\"_blank\">http://dx.doi.org/10.1016/j.scitotenv.2010.04.005</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":1,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"Silvestre, F.; Linares-Casenave, J.; Doroshov, S.I.; Kültz, D.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Silvestre, F. <i>et al.</i>","Englishabstract":"Temperature and selenium are two environmental parameters that potentially affect reproduction and stock recruitment of sturgeon in the San Francisco Bay/Delta Estuary. To identify proteins whose expression is modified by these environmental stressors, we performed a proteomic analysis on larval green and white sturgeons exposed to 18 or 26 °C and micro-injected with Seleno-L-Methionine to reach 8 µg g<sup>-</sup><sup>1</sup> selenium body burden, with L-Methionine as a control. Selenium and high temperature induced mortalities and abnormal morphologies in both species, with a higher mortality in green sturgeon. Larval proteins were separated by two-dimensional gel electrophoresis and differential abundances were detected following spot quantitation and hierarchical cluster analysis. In green sturgeon, 34 of 551 protein spots detected on gels showed a variation in abundance whereas in white sturgeon only 9 of 580 protein spots were differentially expressed (<em>P</em> < 0.01). Gel replicates were first grouped according to heat treatment. Fifteen of these spots were identified using MALDI TOF/TOF mass spectrometry. Proteins involved in protein folding, protein synthesis, protein degradation, ATP supply and structural proteins changed in abundance in response to heat and/or selenium. 40S ribosomal protein SA, FK506-binding protein 10, 65 kDa regulatory subunit A of protein phosphatase 2, protein disulfide isomerase, stress-induced-phosphoprotein 1, suppression of tumorigenicity 13 and collagen type II alpha 1, were differentially expressed in high temperature treatment only. Serine/arginine repetitive matrix protein 1, creatine kinase, serine peptidase inhibitor Kazal type 5 and HSP90 were sensitive to combined temperature and selenium exposure. Valosin-containing protein, a protein involved in aggresome formation and in protein quality control decreased more than 50% in response to selenium treatment. Potential use of such proteins as biomarkers of environmental stressors in larval sturgeons could indicate early warning signals preceding population decline.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A proteomic analysis of green and white sturgeon larvae exposed to heat stress and selenium","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-18 01:31:33.236074","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"    Sturgeon;     Selenium;     Temperature;     Proteomics;     Valosin-containing protein;     Biomarker","OtherDescriptors":null,"Notes":null,"AnaPub":2010,"MonPub":null,"DateUpdate":"2015-11-04","DateCreate":"2012-11-07","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000279773200017","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.scitotenv.2010.04.005"},"refs":null,"anarec":{"AnaID":219848,"PubliDate":2010,"Pagination":"3176-3188","XtraPublOfAnaID":null,"ISBN":null,"Volume":"408","Issue":"16","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43780,"SerRR":"Science of the Total Environment. 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