{"refrec":{"BRefID":220838,"RR":"<b>Willems, M.; Boone, M.; Couvreur, M.; De Mulder, K.; Van Ranst, J.; Artois, T.; Borgonie, G.</b> (2009). Use of freeze-cracking in ontogenetic research in <i>Macrostomum lignano</i> (Macrostomida, Rhabditophora). <i>Dev. Genes Evol. 219(5)</i>: 273-279. <a href=\"https://dx.doi.org/10.1007/s00427-009-0284-x\" target=\"_blank\">https://dx.doi.org/10.1007/s00427-009-0284-x</a>","BEntID":212568,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Dev. Genes Evol. 219(5)</i>: 273-279. <a href=\"https://dx.doi.org/10.1007/s00427-009-0284-x\" target=\"_blank\">https://dx.doi.org/10.1007/s00427-009-0284-x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Willems, M.; Boone, M.; Couvreur, M.; De Mulder, K.; Van Ranst, J.; Artois, T.; Borgonie, G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Willems, M. <i>et al.</i>","Englishabstract":"A method for studying whole mount flatworm embryos based on freeze-cracking of the eggs is described. This method allows successful immunohistological and immunocytological studies of whole mount embryos. It does not require the use of sharpened needles or a microinjection system to puncture the eggshell. Moreover, this method is more practical and less time-consuming than classical puncturing and much cheaper than the use of a microinjection system. The advantages of this method are illustrated by results of several immunolocalisation experiments in the macrostomid flatworm <i>Macrostomum lignano</i>. 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