{"refrec":{"BRefID":222610,"RR":"<b>Nedashkovskaya, O.I.; Van Trappen, S.; Frolova, G.M.; De Vos, P.</b> (2012). <i>Bacillus berkeleyi</i> sp. nov., isolated from the sea urchin <i>Strongylocentrotus intermedius</i>. <i>Arch. Microbiol. 194(3)</i>: 215-221. <a href=\"http://dx.doi.org/10.1007/s00203-011-0771-0\" target=\"_blank\">http://dx.doi.org/10.1007/s00203-011-0771-0</a>","BEntID":214335,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Arch. Microbiol. 194(3)</i>: 215-221. <a href=\"http://dx.doi.org/10.1007/s00203-011-0771-0\" target=\"_blank\">http://dx.doi.org/10.1007/s00203-011-0771-0</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Nedashkovskaya, O.I.; Van Trappen, S.; Frolova, G.M.; De Vos, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Nedashkovskaya, O.I. <i>et al.</i>","Englishabstract":"A bacterial strain, designated KMM 6244T, was isolated from the sea urchin Strongylocentrotus intermedius and subjected to a polyphasic taxonomic investigation. The bacterium was found to be heterotrophic, aerobic, non-motile and spore-forming. Comparative phylogenetic analysis based on 16S rRNA gene sequencing placed the marine isolate in the genus Bacillus. The nearest neighbor of strain KMM 6244T was Bacillus decolorationis LMG 19507T with a 16S rRNA gene sequence similarity of 98.0%. Sequence similarities with the other recognized Bacillus species were less than 96.0%. The results of the DNA–DNA hybridization experiments revealed a low relatedness (37%) of the novel isolate with the type strain of B. decolorationis LMG 19507T. Strain KMM 6244T grew at 4–45°C and with 0–12% NaCl. It produced catalase and oxidase and hydrolyzed aesculin, casein, gelatin and DNA. The predominant fatty acids were anteiso-C15:0, iso-C15:0, anteiso-C17:0, C15:0, iso-C16:0 and iso-C14:0. The DNA G + C content was 39.4 mol%. A combination of phylogenetic, genotypic and phenotypic data clearly indicated that strain KMM 6244T represents a novel species in the genus Bacillus, for which the name Bacillus berkeleyi sp. nov. is proposed. 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