{"refrec":{"BRefID":225235,"RR":"<b>Kirby, R.R.</b> (2000). Cloning and primary structure of putative cytosolic and mitochondrial malate dehydrogenase from the mollusc <i>Nucella lapillus</i> (L.). <i>Gene 245(1)</i>: 81-88. <a href=\"http://dx.doi.org/10.1016/S0378-1119(00)00036-6\" target=\"_blank\">http://dx.doi.org/10.1016/S0378-1119(00)00036-6</a>","BEntID":216958,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Gene 245(1)</i>: 81-88. <a href=\"http://dx.doi.org/10.1016/S0378-1119(00)00036-6\" target=\"_blank\">http://dx.doi.org/10.1016/S0378-1119(00)00036-6</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Kirby, R.R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Kirby, R.R.","Englishabstract":"The evolutionary history of the malate dehydrogenase (<em>MDH</em>) gene family [NAD-dependent <em>MDH</em>; EC 1.1.1.37 and NAD(P)-dependent <em>MDH</em>; EC 1.1.1.82] has received much attention. MDHs have also featured extensively as electrophoretic markers in population genetics and evolutionary ecology, and in many cases, intraspecific variation in MDH has been correlated with environmental variables. However, while the amino acid residues essential for MDH function are known, no studies have examined intraspecific nucleotide variation despite evidence indicating that natural selection may be operating on this locus. This study presents two sets of degenerate oligonucleotide PCR primers to facilitate the cloning of cytosolic <em>MDH</em> (<em>cMDH</em>) and mitochondrial <em>MDH</em> (<em>mMDH</em>) from a broad range of animals (<em>cMDH</em>) and animals and plants (<em>mMDH</em>). These primers were used to obtain putative <em>cMDH</em> and <em>mMDH</em> cDNAs from the mollusc <em>Nucella lapillus</em>. The <em>N. lapillus</em><em>cMDH</em> cDNA was found to encode a putative cMDH protein of 334&#xA0;aa and 36&#xA0;kDa, while the <em>mMDH</em> cDNA encoded a putative mature mMDH protein of 315&#xA0;aa and 33&#xA0;kDa. The putative amino acid sequences of the two compartmentalised <em>N. lapillus</em> MDHs are presented and compared to other known MDH sequences.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Cloning and primary structure of putative cytosolic and mitochondrial malate dehydrogenase from the mollusc <i>Nucella lapillus</i> (L.)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Degenerate; MDH","OtherDescriptors":null,"Notes":null,"AnaPub":2000,"MonPub":null,"DateUpdate":"2013-05-03","DateCreate":"2013-05-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000085869700010","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/S0378-1119(00)00036-6"},"refs":null,"anarec":{"AnaID":225235,"PubliDate":2000,"Pagination":"81-88","XtraPublOfAnaID":null,"ISBN":null,"Volume":"245","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42728,"SerRR":"Gene. ELSEVIER SCIENCE BV: Tokyo; Oxford; New York; Lausanne; Shannon; Amsterdam.  ISSN 0378-1119; e-ISSN 1879-0038","StandardTitleSer":"Gene","ISSN":"0378-1119","AbbrevSer":"Gene","StandardTitleMon":null,"StartPage":81,"Pages":8,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Kirby","Firstname":null,"Initials":"R.R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":216958,"AutID":21213,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":246586,"LibID":36,"BRefID":225235,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"246586","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"ELSEVIER SCIENCE BV","City":"Tokyo; Oxford; New York; Lausanne; Shannon; Amsterdam"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Evolution","ThestID":2944,"Acronym":"ASFA","ThesTermPath":"Biological phenomena > Evolution"},{"ThesaurusTerm":"Invertebrates","ThestID":105118,"Acronym":"CAB","ThesTermPath":"Organisms > Eukaryotes > Animals > Invertebrates"}],"thestermsASFA":[{"ThesaurusTerm":"Evolution"},{"ThesaurusTerm":"Invertebrates"}],"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":216958,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"http://dx.doi.org/10.1016/S0378-1119(00)00036-6","externalID":"10.1016/S0378-1119(00)00036-6","URLTypeCode":"DOI","URLID":26929,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Evolution","ThestID":2944,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Invertebrates","ThestID":105118,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Evolution","DutchTerm":"Evolutie","ThestID":2944,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Invertebrates","DutchTerm":null,"ThestID":105118,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2013-05-03 10:33:51.443000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2013-05-03 10:33:51.443000","timezone_type":3,"timezone":"Europe/Brussels"}}}