{"refrec":{"BRefID":2264,"RR":"<b>Sasaki, K.; Matsumoto, G.; Takashima, K.; Fujita, R.; Kawasaki, K.; Kimura, D.K.; Sato, M.</b> (1994). Depressing effects of protein kinases A & C on the receptor- induced K<sup>+</sup>-current responses in the ganglion cells of <i>Aplysia</i>. <i>Neth. J. Zool. 44(3-4)</i>: 578-587","BEntID":2264,"PublicFlag":1,"CheckedFlag":0,"wosflag":null,"vabbflag":null,"RefStringPartII":". <i>Neth. J. Zool. 44(3-4)</i>: 578-587","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Sasaki, K.; Matsumoto, G.; Takashima, K.; Fujita, R.; Kawasaki, K.; Kimura, D.K.; Sato, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Sasaki, K. <i>et al.</i>","Englishabstract":"Application of either dopamine (DA), acetylcholine (ACh), histamine (Ha), or Phe-Met-Arg-Phe-NH<sub>2</sub> (FMRFamide) induces a K<sup>+</sup>-current response in the ganglion cells of <i>Aplysia</i> under voltage clamp. We have previously reported that these responses are all mediated by a pertussis toxin (PTX)-sensitive G-protein Gi or Go. Intracellular application of cAMP, an activator of protein kinase A (PKA), or extracellular application of 30 µM phorbol dibutyrate (PDBu), an activator of protein kinase C (PKC), markedly suppressed these transmitter-induced K<sup>+</sup>-current responses. The depressing effect of cAMP was reversible while that of PDBu irreversible as observed for 1 hour. Intracellular injection of okadaic acid (OA), an inhibitor phosphatases, facilitated the blocking effects of both cAMP and PDBu. The dose-response curve obtained by each transmitter-receptor system shifted downward by application of either cAMP or PDBu without affecting the affinity of the agonist to each receptor. K<sup>+</sup>-channel opening directly induced by guanosine thiotriphosphate (GTPgammaS) or by raising the temperature was not depressed y either cAMP or 100 µM PDBu. From these results, we postulated that the acting sites of both PKA and PKC might be somewhere between the receptors and G-protein, and that the phosphorylation of these sites would the functional coupling efficiency between the receptors and G-protein.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Depressing effects of protein kinases A & C on the receptor- induced K<sup>+</sup>-current responses in the ganglion cells of <i>Aplysia</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1994,"MonPub":null,"DateUpdate":"2003-01-07","DateCreate":"2000-08-24","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":2264,"PubliDate":1994,"Pagination":"578-587","XtraPublOfAnaID":null,"ISBN":null,"Volume":"44","Issue":"3-4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43566,"SerRR":"Netherlands Journal of Zoology. 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