{"refrec":{"BRefID":226680,"RR":"<b>Becker, P.; Gillan, D.C.; Eeckhaut, I.</b> (2009). Characterization of the bacterial community associated with body wall lesions of <i>Tripneustes gratilla</i> (Echinoidea) using culture-independent methods. <i>J. Invertebr. Pathol. 100(2)</i>: 127-130. <a href=\"http://dx.doi.org/10.1016/j.jip.2008.11.002\" target=\"_blank\">http://dx.doi.org/10.1016/j.jip.2008.11.002</a>","BEntID":218398,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>J. Invertebr. Pathol. 100(2)</i>: 127-130. <a href=\"http://dx.doi.org/10.1016/j.jip.2008.11.002\" target=\"_blank\">http://dx.doi.org/10.1016/j.jip.2008.11.002</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Becker, P.; Gillan, D.C.; Eeckhaut, I.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Becker, P. <i>et al.</i>","Englishabstract":"The bacterial community associated with skin lesions of the sea urchin <i>Tripneustes gratilla</i> was investigated using 16S ribosomal RNA gene cloning and fluorescent <i>in situ</i> hybridization (FISH). All clones were classified in the <i>Alphaproteobacteria</i>, <i>Gammaproteobacteria</i> and <i>Cytophaga</i>-<i>Flexibacter</i>-<i>Bacteroides</i> (CFB) bacteria. Most of the <i>Alphaproteobacteria</i> were related to the <i>Roseobacter</i> lineage and to bacteria implicated in marine diseases. The majority of the <i>Gammaproteobacteria</i> were identified as <i>Vibrio</i> while CFB represented only 9% of the total clones. FISH analyses showed that <i>Alphaproteobacteria</i>, CFB bacteria and <i>Gammaproteobacteria</i> accounted respectively for 43%, 38% and 19% of the DAPI counts. 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