{"refrec":{"BRefID":226716,"RR":"<b>Léonet, A.; Rasolofonirina, R.; Wattiez, R.; Jangoux, M.; Eeckhaut, I.</b> (2009). A new method to induce oocyte maturation in holothuroids (Echinodermata). <i>Invertebr. Reprod. Dev. 53(1)</i>: 13-21. <a href=\"https://dx.doi.org/10.1080/07924259.2009.9652285\" target=\"_blank\">https://dx.doi.org/10.1080/07924259.2009.9652285</a>","BEntID":218434,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Invertebr. Reprod. Dev. 53(1)</i>: 13-21. <a href=\"https://dx.doi.org/10.1080/07924259.2009.9652285\" target=\"_blank\">https://dx.doi.org/10.1080/07924259.2009.9652285</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Léonet, A.; Rasolofonirina, R.; Wattiez, R.; Jangoux, M.; Eeckhaut, I.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Léonet, A. <i>et al.</i>","Englishabstract":"Oocyte maturation in sea cucumbers is stopped during the meiosis at prophase I. Maturation naturally concludes just before spawning leading to a mature oocyte ready to be fertilized. Although thermal shocks applied on mature individuals can induce spawning, it is currently not possible to perform reliably <i>in vitro</i> fertilization of sea cucumber oocytes. The present paper reports the discovery of a new and highly effective oocyte maturation inducer (OMI), called MIF (for maturation inducing fractions). MIF's effects on oocytes of the marketable species <i>Holothuria</i> scabra were analysed and compared to that of the few OMIs described in literature. MIF induces the maturation and fertilization of more than 90% of oocytes while other OMIs [1-methyladenine, dithiothreitol (DTT), dimercapto-propanol (BAL) and L-cysteine] induces 28–90% of oocytes to mature depending on the tested inducer. Moreover the use of the other OMIs result in fertilization rates that never exceed 40%, and the obtained larvae often resent developmental abnormalities. MIF's action on H. scabra oocytes is efficient yearlong even outside the spawning period of <i>H. scabra</i>. MIF is effective on the oocytes of all aspidochirote species tested so far but only when it is prepared from spawns of regular female sea urchin.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A new method to induce oocyte maturation in holothuroids (Echinodermata)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-22 01:31:45.196968","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"    Sea cucumber,     holothuriculture,     oocyte,     maturation,     MIF","OtherDescriptors":null,"Notes":null,"AnaPub":2009,"MonPub":null,"DateUpdate":"2018-08-17","DateCreate":"2013-06-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000269224700002","VABBcode":null,"OpenAcc":0,"DOI":"10.1080/07924259.2009.9652285"},"refs":null,"anarec":{"AnaID":226716,"PubliDate":2009,"Pagination":"13-21","XtraPublOfAnaID":null,"ISBN":null,"Volume":"53","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43172,"SerRR":"Invertebrate Reproduction & Development. 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