{"refrec":{"BRefID":229438,"RR":"<b>Dupont, A.; Siebert, U.; Covaci, A.; Weijs, L.; Eppe, G.; Debier, C.; De Pauw-Gillet, M.-C.; Das, K.</b> (2013). Relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (<i>Phoca vitulina</i>) from the North Sea. <i>Aquat. Toxicol. 142-143</i>: 210-220. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2013.08.014\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2013.08.014</a>","BEntID":221159,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquat. Toxicol. 142-143</i>: 210-220. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2013.08.014\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2013.08.014</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Dupont, A.; Siebert, U.; Covaci, A.; Weijs, L.; Eppe, G.; Debier, C.; De Pauw-Gillet, M.-C.; Das, K.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Dupont, A. <i>et al.</i>","Englishabstract":"<i>In vitro</i> culture of peripheral blood leucocytes (PBLs) is currently used in toxicological studies of marine mammals. However, blood cells of wild individuals are exposed <i>in vivo</i> to environmental contaminants before being isolated and exposed to contaminants <i>in vitro</i>. The aim of this study was to highlight potential relationships between blood contaminant levels and <i>in vitro</i> peripheral blood lymphocyte proliferation in free-ranging adult harbour seals (<i>Phoca vitulina</i>) from the North Sea. Blood samples of 18 individuals were analyzed for trace elements (Fe, Zn, Se, Cu, Hg, Pb, Cd) and persistent organic contaminants and metabolites (SPCBs, SHO-PCBs, SPBDEs, 2-MeO-BDE68 and 6-MeO-BDE47, SDDXs, hexachlorobenzene, oxychlordane, <i>trans</i>-nonachlor, pentachlorophenol and tribromoanisole). The same samples were used to determine the haematology profiles, cell numbers and viability, as well as the <i>in vitro</i> ConA-induced lymphocyte proliferation expressed as a stimulation index (SI). Correlation tests (Bravais-Pearson) and Principal Component Analysis with multiple regression revealed no statistically significant relationship between the lymphocyte SI and the contaminants studied. However, the number of lymphocytes per millilitre of whole blood appeared to be negatively correlated to pentachlorophenol (<i>r</i> = -0.63, <i>p</i> = 0.005). In adult harbour seals, the interindividual variations of <i>in vitro</i> lymphocyte proliferation did not appear to be directly linked to pollutant levels present in the blood, and it is likely that other factors such as age, life history, or physiological parameters have an influence. In a general manner, experiments with <i>in vitro</i> immune cell cultures of wild marine mammals should be designed so as to minimize confounding factors in which case they remain a valuable tool to study pollutant effects <i>in vitro</i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (<i>Phoca vitulina</i>) from the North Sea","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-19 01:31:34.951598","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Lymphoproliferation; Immune parameters","OtherDescriptors":null,"Notes":null,"AnaPub":2013,"MonPub":null,"DateUpdate":"2019-06-25","DateCreate":"2013-10-02","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000328093900021","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2013.08.014"},"refs":null,"anarec":{"AnaID":229438,"PubliDate":2013,"Pagination":"210-220","XtraPublOfAnaID":null,"ISBN":null,"Volume":"142-143","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam.  ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":210,"Pages":11,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Dupont","Firstname":"Aurélie","Initials":"A.","Affiliation":"Laboratory of Oceanology – MARE Center, University of Liège","Discriminator":null,"CorporateFlag":0,"BEntID":221159,"AutID":165230,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Université de Liège; Faculté des Sciences; Département de Biologie, Ecologie et Evolution; Laboratoire d'Océanologie Biologique","ORCID":null,"PersID":28748,"InsID":459},{"AutName":"Siebert","Firstname":"Ursula","Initials":"U.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":221159,"AutID":11049,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Covaci","Firstname":"Adrian","Initials":"A.","Affiliation":"Toxicological Centre, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":221159,"AutID":149632,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Antwerpen; Faculteit Farmaceutische, Biomedische en Diergeneeskundige Wetenschappen; Toxicologisch Centrum","ORCID":"0000-0003-0527-1136","PersID":2633,"InsID":111},{"AutName":"Weijs","Firstname":"Liesbeth","Initials":"L.","Affiliation":"Toxicological Centre, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":221159,"AutID":148906,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"SPHERE","InsFSN":"Universiteit Antwerpen; Faculteit Wetenschappen; Departement Biologie; Onderzoeksgroep Systemisch Fysiologisch en Ecotoxicologisch Onderzoek","ORCID":"0000-0002-2399-1711","PersID":16339,"InsID":108},{"AutName":"Eppe","Firstname":"Gauthier","Initials":"G.","Affiliation":"Center for Analytical Research and Technology (CART), Inorganic Analytical Chemistry, University of Liège","Discriminator":null,"CorporateFlag":0,"BEntID":221159,"AutID":165233,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"CART","InsFSN":"Université de Liège; Center for Analytical Research and Technology","ORCID":null,"PersID":4691,"InsID":13949},{"AutName":"Debier","Firstname":"Cathy","Initials":"C.","Affiliation":"Unit of Nutrition Biochemistry, Catholic University of Leuven","Discriminator":null,"CorporateFlag":0,"BEntID":221159,"AutID":165234,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"BNTE","InsFSN":"Université Catholique de Louvain; Louvain Institute of Biomolecular Science and Technology; Biochemistry of Nutrition and Environmental Toxicology","ORCID":null,"PersID":7069,"InsID":3835},{"AutName":"De Pauw-Gillet","Firstname":"Marie-Claire","Initials":"M.-C.","Affiliation":"Mammalian Cell Culture Laboratory (GIGA-R), University of Liège","Discriminator":null,"CorporateFlag":0,"BEntID":221159,"AutID":165235,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Das","Firstname":"Krishna","Initials":"K.","Affiliation":"Laboratory for Oceanology-MARE Center, University of Liège","Discriminator":null,"CorporateFlag":0,"BEntID":221159,"AutID":146017,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Université de Liège; Faculté des Sciences; Département de Biologie, Ecologie et Evolution; Laboratoire d'Océanologie Biologique","ORCID":"0000-0003-2689-3348","PersID":3862,"InsID":459}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":251270,"LibID":36,"BRefID":229438,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"251270","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open Marien Archief","URL":null,"ClassifID":235,"Classification":"Open Marine Archive","ReqLink":null,"ClassifTypID":5,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":0,"InternalReq":0,"LoggedInReq":0,"Disclaimer":"Disclaimer_OMA","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier Science","City":"Tokyo; New York; London; Amsterdam"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Blood","ThestID":1050,"Acronym":"ASFA","ThesTermPath":"Fluids > Body fluids > Blood"},{"ThesaurusTerm":"Contaminants","ThestID":106723,"Acronym":"CAB","ThesTermPath":"Products > Contaminants"},{"ThesaurusTerm":"In vitro","ThestID":137549,"Acronym":"CAB","ThesTermPath":"Techniques > In vitro"}],"thestermsASFA":[{"ThesaurusTerm":"Blood"},{"ThesaurusTerm":"Contaminants"},{"ThesaurusTerm":"In vitro"}],"taxtermsASFA":[{"TaxTerm":"Phoca vitulina"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":221159,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":221159,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.aquatox.2013.08.014","externalID":"10.1016/j.aquatox.2013.08.014","URLTypeCode":"DOI","URLID":77109,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Blood","ThestID":1050,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Contaminants","ThestID":106723,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"In vitro","ThestID":137549,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxterms":[{"TaxTerm":"Phoca vitulina","AphiaID":137084,"TaxtID":39543}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Blood","DutchTerm":"Bloed","ThestID":1050,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Contaminants","DutchTerm":null,"ThestID":106723,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"In vitro","DutchTerm":null,"ThestID":137549,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxtermsFRIS":[{"TaxTerm":"Phoca vitulina","DutchTerm":null,"AphiaID":137084,"TaxtID":39543}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2013-10-02 08:44:40.430000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2019-06-25 09:53:20.810000","timezone_type":3,"timezone":"Europe/Brussels"}}}