{"refrec":{"BRefID":229552,"RR":"<b>Fernández-Tajes, J.; Méndez, J.</b> (2007). Identification of the razor clam species <i>Ensis arcuatus, E. siliqua, E. directus, E. macha</i>, and <i>Solen marginatus</i> using PCR-RFLP analysis of the 5S rDNA region. <i>J. Agric. Food Chem. 55(18)</i>: 7278-7282. <a href=\"http://dx.doi.org/10.1021/jf0709855\" target=\"_blank\">http://dx.doi.org/10.1021/jf0709855</a>","BEntID":221273,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. Agric. Food Chem. 55(18)</i>: 7278-7282. <a href=\"http://dx.doi.org/10.1021/jf0709855\" target=\"_blank\">http://dx.doi.org/10.1021/jf0709855</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Fernández-Tajes, J.; Méndez, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Fernández-Tajes, J.; Méndez, J.","Englishabstract":"Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis of the 5S ribosomal DNA region has been applied to the establishment of DNA-based molecular markers for the identification of five razor clam species:? <i>Ensis arcuatus, E. siliqua, E. directus, E. macha</i>, and <i>Solen marginatus</i>. PCR amplifications were carried out using a pair of universal primers from the coding region of 5S rDNA. <i>S. marginatus</i> was simply distinguished by the different size of the amplicons obtained. Species-specific restriction endonuclease patterns were found with the enzymes Hae III for <i>E. arcuatus, E. siliqua</i>, and <i>E. directus</i>, and Acs I for <i>E. macha</i>, and when two enzymes were combined, the four species were also identified. Thus, this work provides a simple, reliable, and rapid protocol for the accurate identification of Ensis and Solen species in fresh and canned products, which is very useful for traceability and to enforce labeling regulations.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Identification of the razor clam species <i>Ensis arcuatus, E. siliqua, E. directus, E. macha</i>, and <i>Solen marginatus</i> using PCR-RFLP analysis of the 5S rDNA region","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-09 01:31:27.156550","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Species identification; Razor clam; Ensis spp; 5S rDNA marker; PCR-RFLP  ","OtherDescriptors":null,"Notes":null,"AnaPub":2007,"MonPub":null,"DateUpdate":"2013-10-07","DateCreate":"2013-10-07","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000249150900009","VABBcode":null,"OpenAcc":0,"DOI":"10.1021/jf0709855"},"refs":null,"anarec":{"AnaID":229552,"PubliDate":2007,"Pagination":"7278-7282","XtraPublOfAnaID":null,"ISBN":null,"Volume":"55","Issue":"18","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43013,"SerRR":"Journal of Agricultural and Food Chemistry. American Chemical Society: Easton, Pa.,.  ISSN 0021-8561; e-ISSN 1520-5118","StandardTitleSer":"Journal of Agricultural and Food Chemistry","ISSN":"0021-8561","AbbrevSer":"J. Agric. Food Chem.","StandardTitleMon":null,"StartPage":7278,"Pages":5,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Fernández-Tajes","Firstname":"Juan","Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":221273,"AutID":79904,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Méndez","Firstname":"Josefina","Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":221273,"AutID":79906,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":251400,"LibID":36,"BRefID":229552,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"251400","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"American Chemical Society","City":"Easton, Pa.,"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Traceability","ThestID":166669,"Acronym":"CAB","ThesTermPath":"Characteristics > Traceability"},{"ThesaurusTerm":"Seafood","ThestID":7309,"Acronym":"ASFA","ThesTermPath":"Food > Human food > Seafood"},{"ThesaurusTerm":"Identification","ThestID":4228,"Acronym":"ASFA","ThesTermPath":"Identification"},{"ThesaurusTerm":"Species","ThestID":7867,"Acronym":"ASFA","ThesTermPath":"Taxa > Species"}],"thestermsASFA":[{"ThesaurusTerm":"Identification"},{"ThesaurusTerm":"Seafood"},{"ThesaurusTerm":"Species"},{"ThesaurusTerm":"Traceability"}],"taxtermsASFA":[{"TaxTerm":"Ensis"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":221273,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"http://dx.doi.org/10.1021/jf0709855","externalID":"10.1021/jf0709855","URLTypeCode":"DOI","URLID":29632,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Identification","ThestID":4228,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Seafood","ThestID":7309,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Species","ThestID":7867,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Traceability","ThestID":166669,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxterms":[{"TaxTerm":"Ensis","AphiaID":138333,"TaxtID":40068}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Identification","DutchTerm":"Identificatie","ThestID":4228,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Seafood","DutchTerm":null,"ThestID":7309,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Species","DutchTerm":"Soorten","ThestID":7867,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Traceability","DutchTerm":null,"ThestID":166669,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxtermsFRIS":[{"TaxTerm":"Ensis","DutchTerm":null,"AphiaID":138333,"TaxtID":40068}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2013-10-07 10:25:36.293000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2013-10-07 10:25:36.293000","timezone_type":3,"timezone":"Europe/Brussels"}}}