{"refrec":{"BRefID":231009,"RR":"<b>Silsbe, G.M.; Kromkamp, J.C.</b> (2012). Modeling the irradiance dependency of the quantum efficiency of potosynthesis. <i>Limnol. Oceanogr., Methods 10</i>: 645-652. <a href=\"http://dx.doi.org/10.4319/lom.2012.10.645\" target=\"_blank\">dx.doi.org/10.4319/lom.2012.10.645</a>","BEntID":222721,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Limnol. Oceanogr., Methods 10</i>: 645-652. <a href=\"https://dx.doi.org/10.4319/lom.2012.10.645\" target=\"_blank\">https://dx.doi.org/10.4319/lom.2012.10.645</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Silsbe, G.M.; Kromkamp, J.C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Silsbe, G.M.; Kromkamp, J.C.","Englishabstract":"Measures of the quantum efficiency of photosynthesis (phi(PSII)) across an irradiance (E) gradient are an increasingly common physiological assay and alternative to traditional photosynthetic-irradiance (PE) assays. Routinely, the analysis and interpretation of these data are analogous to PE measurements. Relative electron transport rates (rETR = E x phi(PSII)) are computed and fit to a PE curve to retrieve physiologically meaningful PE parameters. This widespread approach is statistically flawed as the response variable (rETR) is explicitly dependent on the predictor variable (E). Alternatively the E-dependency of phi(PSII) can be modeled directly while retaining the desired PE parameters by normalizing a given PE model to E. This manuscript presents a robust analysis in support of this alternative procedure. First, we demonstrate that scaling phi(PSII) to rETR unnecessarily amplifies the measurement error of phi(PSII) and using a Monte-Carlo analysis on synthetic data induces significantly higher uncertainty in computed PE parameters relative to modeling the E-dependency of phi(PSII) directly. Next a large dataset is simultaneously fitted to four PE models implemented in their original and E-normalized forms. Four statistical criteria used to evaluate the efficacy of nonlinear models demonstrate improved model fits and more precise PE parameters when data are modeled as E-dependent changes in phi(PSII). The analysis presented in this manuscript clearly demonstrates that modeling the E-dependency of phi(PSII) directly should be the norm for interpreting active fluorescence measures.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Modeling the irradiance dependency of the quantum efficiency of potosynthesis","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2012,"MonPub":null,"DateUpdate":"2016-10-26","DateCreate":"2013-12-01","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000310052700003","VABBcode":null,"OpenAcc":1,"DOI":"10.4319/lom.2012.10.645"},"refs":null,"anarec":{"AnaID":231009,"PubliDate":2012,"Pagination":"645-652","XtraPublOfAnaID":null,"ISBN":null,"Volume":"10","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":75192,"SerRR":"Limnology and Oceanography: Methods. 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